Lipopolysaccharide upregulates the expression of Toll-like receptor 4 in human vascular endothelial cells  

内毒素上调血管内皮细胞TLR4m RNA的表达(英文)

作　　者：杨清武[1] 朱佩芳[1] 王正国[1] 蒋建新[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所四室,重庆400042

出　　处：《Chinese Medical Journal》2002年第2期286-289,158,共4页中华医学杂志（英文版）

基　　金：theMajorStateBasicResearchDevelopmentProgramofChina (No G19990 5 42 0 3)

摘　　要：OBJECTIVE: To investigate the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) and the effect of lipopolysaccharide (LPS) on their expression in cultured endothelial cells. METHODS: Total RNA was extracted from ECV304 cells and isolated human umbilical vein endothelial cells (HUVECs) exposed to LPS, respectively. The quantification of TLR2 and TLR4 mRNA in HUVECs and EVC304 cells was carried out by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: ECV304 cells and HUVECs were able to express TLR2 and TLR4 mRNA, but the expression levels of TLR4 appeared to be stronger than those of TLR2. LPS could upregulate the expression levels of TLR4 obviously, whereas it had no effect on the expression level of TLR2. CONCLUSIONS: Our data indicate that TLR4 may be the LPS signal transducer in endothelial cells and plays important roles in the cell activation of LPS. The ECV304 cell line is a better experimental model than isolated HUVECs in the research of endothelial cells.目的　探讨内皮细胞TLR2和TLR4的表达以及内毒素对其表达的影响。方法　按RNA提取试剂盒说明书提取内毒素作用后的ECV 30 4 (人脐静脉内皮细胞株 )和人脐静脉内皮细胞总RNA ,运用逆转录PCR(RT PCR)检测细胞TLR2和TLR4mRNA的表达。结果　人脐静脉内皮细胞和ECV30 4细胞株均能表达TLR2和TLR4mRNA ,但是 ,TLR4的表达水平明显强于TLR2的表达 ,LPS能明显上调TLR4的表达 ,但对TLR2的表达无影响。结论　本研究提示TLR4可能内皮细胞上LPS作用的信号转导分子 ,在LPS对内皮细胞的激活效应中具有重要的作用 ;ECV 30 4是内皮细胞研究的良好的实验模型 ,可代替人脐静脉内皮细胞而较为广泛应用。

关 键 词：Drosophila Proteins Cell Line Dose-Response Relationship  Drug Endothelium  Vascular Gene Expression Regulation Humans LIPOPOLYSACCHARIDES Membrane Glycoproteins RNA  Messenger Receptors  Cell Surface Research Support  Non-U.S. Gov't Time Factors Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptors Umbilical Veins UP-REGULATION 

分 类 号：R540.2[医药卫生—心血管疾病]