Comparative study of catheter-mediated gene transfer into heart  

导管介导基因转导心脏的对比研究

作　　者：李建军[1] 李庚山[1] 黄从新[1] 江洪[1] 唐基柱[1] 许家俐[1] 夏豪[1] 王晶[1] 

机构地区：[1]武汉大学人民医院心内科,武汉,430060

出　　处：《Chinese Medical Journal》2002年第4期612-613,共2页中华医学杂志（英文版）

基　　金：教育部留学回国人员科研启动基金

摘　　要：OBJECTIVE: To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS: Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis. RESULTS: The results showed that (1) the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; (2) gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; (3) LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium. CONCLUSION: Three catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.目的探讨经自制针型导管心肌内注射(简称心肌注射法)、经冠状动脉灌注(简称冠脉灌注法)及经心房穿刺心包(简称心包注射法)等三种方法导基因入心脏的可行性及利与弊.方法选成年杂交犬15只,将含有同等剂量(1×109/ml pfu)携带有细菌LacZ基因的重组腺病毒载体(Adex1SRLacZ),经上述三种方法导基因入心脏.术后5天处死动物,细织化学法分析Lac Z基因在心包及心肌中的表达.结果 (1)冠脉灌注法导基因入心脏后,LacZ基因在心肌中呈点或小片状表达;心肌注射法导基因入心脏后,LacZ基因沿注射轨迹成块或片状表达;心包注射法导基因入心包后,犬心包脏层、壁层及脏层下心肌均可见散在的LacZ基因表达,而使用同样方法注射对照腺病毒Adex lw 入犬心肌及心包均未见LacZ基因表达.(2)心肌注射法导基因入心脏的同时,心肌中可见明显的炎性细胞浸润,而冠脉灌注法及心包注射法导基因入心脏后则未见炎性细胞浸润.(3) 冠脉灌注法导基因入心脏的同时,肝脏细织中检测有散在的LacZ基因表达,而心肌注射法及心包注射法则不然.结论心肌注射法、冠脉灌注法及心包注射法是导基因入心脏的可行性方法,各有利弊,应用时可根据研究目的合理选用.

关 键 词：Gene Transfer Techniques ADENOVIRIDAE Animals Comparative Study Dogs Female Gene Expression Genetic Vectors Heart Catheterization Inflammation INJECTIONS Lac Operon Liver Male MYOCARDIUM Research Support  Non-U.S. Gov't 

分 类 号：R54[医药卫生—心血管疾病]