Retinal dopamine transporter in experimental myopia  

实验性近视眼视网膜多巴胺转运蛋白研究(英文)

作　　者：席晓勍[1] 褚仁远[2] 周行涛[2] 卢奕[2] 刘兴党[3] 

机构地区：[1]复旦大学附属中山医院眼科,上海200032 [2]复旦大学附属眼耳鼻喉科医院眼科,上海200031 [3]复旦大学附属华山医院核医学科,上海200031

出　　处：《Chinese Medical Journal》2002年第7期1027-1030,149,共4页中华医学杂志（英文版）

基　　金：ThisworkwassupportedbytheKeyProjectofClinicalScienceoftheHealthyMinistryofChina (No 970 3 0 2 2 5 )

摘　　要：OBJECTIVE: To investigate the distribution, changes and a possible role for retinal dopamine transporter (DAT) in experimental myopia in chickens. METHODS: Two-day-old chickens were divided into four groups. Chicken eyes were fitted with lenses of -10D,-20D and translucent goggles unilaterally. Normal eyes were used as controls. After 3 wk, all chickens were given an intramuscular injection of (125)I-beta-CIT 2beta-carbomethoxy-3beta-(4-iodophenyl)tropane and sacrificed two hours post injection. Retinal pigment epithelium (RPE) and the neural retina were obtained together or RPE was dissected out from the neural retina. Radioactive DAT from each specimen was assayed by gamma-counter. RESULTS: Retinal DAT was detected in RPE specimens rather than in the neural retina in all eyes. Radioactive DAT in myopic eyes was higher, compared with control eyes. CONCLUSIONS: Retinal DAT is mainly located in the RPE and may be involved in the formation of lens induced myopia (LIM) and form deprivation myopia (FDM). These methods may provide a new approach for further studying the role of the dopamine system in experimental myopia.目的　研究鸡眼视网膜多巴胺转运蛋白 (dopaminetransporter ,DAT)的分布及其在实验性近视眼形成中的变化及可能作用。方法　孵化第 2天的雏鸡随机分为 4组 ,Ⅰ、Ⅱ、Ⅲ组小鸡单眼分别戴 10D、 2 0D透镜及半透明眼罩 ,IV组为正常对照组。 3周后测量双眼屈光状态及眼轴长度。一侧大腿肌注12 5I β CIT ,二小时后处死小鸡 ,摘出眼球 ,解剖出全层视网膜组织 (Ⅰ及Ⅳ组 )或视网膜神经上皮层与视网膜色素上皮层 (retinalpigmentepithelium ,RPE)分别取下(Ⅱ及Ⅲ组 ) ,以β 计数器分别测定各组织标本多巴胺转运蛋白的放射性计数。结果　RPE组织标本检测到的DAT放射性计数明显高于视网膜神经上皮层。实验眼视网膜组织标本DAT放射性计数大于对照眼。结论　视网膜DAT主要分布在RPE。视网膜DAT可能参与形觉剥夺性近视及透镜诱导性近视的形成。这种研究方法为今后深入研究多巴胺系统与实验性近视眼的关系提供了一条新的途径。

关 键 词：Membrane Glycoproteins Nerve Tissue Proteins ANIMALS CHICKENS Dopamine Plasma Membrane Transport Proteins Eye Membrane Transport Proteins MYOPIA Research Support  Non-U.S. Gov't RETINA 

分 类 号：R778.11[医药卫生—眼科]