多种神经递质参与外周跨节段信息传递  

Multiple Neuropeptides Participate in the Peripheral Information Transmission across Dermatomes

作　　者：赵晏[1] 孙启新[1] 张世红[1] 贾军[1] 曹东元[1] 郭媛[1] 

机构地区：[1]西安交通大学医学院生理教研室,陕西710061

出　　处：《针刺研究》2002年第4期298-302,共5页Acupuncture Research

基　　金："九.五"国家攀登计划资助项目 (JL930 2 1和 95 -预 - 19- 131);陕西省中医局资助项目 (2 0 0 1- 12 )

摘　　要：目的 :研究参与外周跨节段信息传递的多种神经递质及其作用机制 ,为阐明经络实质提供资料。方法 :在大鼠足太阳膀胱经上刺激一个穴位的支配神经 ,用电生理学方法在相邻节段或者相隔多个节段的穴位支配神经上观察放电数的变化。在跨节段信息传递的模型上 ,使用辣椒素损伤肽能神经 ,并应用P物质 (SP)、降钙素基因相关肽 (CGRP)、神经激肽A(NKA)的激动剂和NK 1受体、H1 受体拮抗剂观察对它们的影响。结果 :首次证实了模拟穴位刺激可引起沿经脉线的跨节段信息传递 ,这种跨节段信息传递现象符合人体循经感传的特性 ,可能是循经感传的生理学基础。用辣椒素处理破坏肽能神经纤维后 ,跨节段信息传递明显减少。SP、NKA和CGRP均使跨节段信息传递易化 ,三种神经肽的作用强度和持续时间为 :SP >CGRP >NKA。应用NK 1受体阻断剂WIN51 ,70 8、H1 受体拮抗剂苯海拉明则可使跨节段信息传递减少。结论 :SP、CGRP、NKA、组织胺等 ,通过与相应的受体结合 ,介导外周跨节段的信息传递。交感神经的激活也可能促进这种信息传递。提示循经感传是由多种递质和受体介导的 ,并且可以从经脉线上直接采集电信号作为研究经络活动的指标。To study the neuropeptides involved in the peripheral information transmission across dermatomes and the relevant mechanisms. Methods: Wistar rats are used in this study. Stimulate one nerve innervating a acupoint of 'Pangguang Meridian of Foot Taiyang' antidromically and record the discharge of the other nerve innervating the neighboring acupoint next to or several segments away from the stimulated nerve. Using the model of the information transmission across dermatomes, the discharges from the acupoint are observed by treatment respectively with capcaisin (destroying the peptidergic nerves), substance P (SP), calcitonin gene related peptide (CGRP), neuropeptide A (NKA) and NK 1 receptor antagonist and H 1 receptor antagonist. Results: This study proves for the first time that imitating acupoint stimulation may induce information transmission across dermatomes along the route of meridian. The characteristics of the information transmission across dermatomes is accord with regularities of propagation of sensation along meridians (PSM), that may contribute to the phenomenon of PSM in clinic. The information transmitted across dermatomes decreased significantly after treatment with capcaisin to destroy the peptidergic nerves. SP, CGRP and NKA facilitated the information transmission across dermatomes and the intensity and duration of the effect are SP>CGRP>NKA. NK 1 receptor antagonist (WIN 51,708) and H 1 receptor antagonist (benzhydramine) decrease the information transmitted across dermatomes. These results suggest that SP, CGRP, NKA, histamine, etc. may contribute to the information transmission across dermatomes via combining with their receptors. The activation of sympathetic nerve may enhance the information transmission. Conclusion: Multiple types of neuropeptides and their receptors are involved in PSM.

关 键 词：外周跨节段信息传递 穴位刺激 神经递质 循经感传 作用机制 经络学 

分 类 号：R224.1[医药卫生—中医基础理论]