中国人胃癌染色体11_p15.5处杂合性缺失分析  

Loss of Heterozygosity on Chromosome 11_p15.5 in Gastric Carcinoma in China

作　　者：刘韬韬[1] 史耀舟[2] 沈锡中[1] 孔祥银[3] 王吉耀[1] 

机构地区：[1]复旦大学附属中山医院消化科,上海200032 [2]中国科技大学生命科学院,合肥230027 [3]中国科学院上海生物工程研究中心,上海200233

出　　处：《复旦学报（医学版）》2003年第1期24-26,29,共4页Fudan University Journal of Medical Sciences

摘　　要：目的　通过对中国人胃癌染色体 11p15 .5处杂合性缺失的研究 ,了解中国人群中胃癌病人在此区域杂合性缺失的情况。方法　从 66例胃癌病人的石蜡包埋的手术病理标本中 ,提取肿瘤及相应正常组织的DNA ,用荧光标记的方法选取 11p15 .5处的微卫星DNA标记进行PCR扩增 ,再将PCR产物进行变性聚丙烯酰胺凝胶电泳 ,电泳后行杂合性缺失分析。结果　 11/ 3 6( 3 0 .6% )的病人在D11S13 18处存在杂合性缺失 ;而D11S40 46位点处 ,13 / 60 ( 2 1.7% )的病例存在杂合性缺失 ;2 4/ 61( 3 9.3 % )的病例在 11p15 .5处至少有 1个位点存在杂合性缺失。杂合性缺失的频率与肿瘤病人的年龄、性别、肿瘤大小、肿瘤部位及淋巴结是否转移均无关。结论　高频杂合性缺失的区域可能有抑癌基因的存在。这将有助于阐明胃癌发生发展的分子机制 ,进而为对胃癌进行风险预测。Purpose: To study the loss of heterozygosity on chromosome 11p15.5 in gastric carcinoma so that we can explore the possible gastric carcinoma related genes of loci. Methods: Dissection of neoplastic and nonneoplastic tissue was performed from histologic sections of 66 gastric carcinomas. After DNA extraction, polymerase chain amplification products of two polymorphic microsatellite markers on chromosome 11p15.5 were analyzed by polyacrylamide gel electrophoresis. Results: Eleven of 36(30.6%) cases were detected for LOH on loci D11S1318.13 of 60(21.7%) cases for that loci D11S4046.24 OF 61(39.3%) cases were detected for at least one loci LOH on chromosome 11p15.5 Occurrence of LOH was not correlated with sex, age, tumor size, tumor site or lymph node metastasis. Conclusions: High frequency LOH regions identified here may point to major susceptibility genes including potential tumor suppressor genes and inherited gene loci, which will assist in understanding the molecular events involved in gastric carcinogenesis and may help in development of markers for genetic susceptibility testing and screening for the early detection of this cancer.

关 键 词：中国人 染色体 杂合性缺失 抑癌基因 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]