茶多酚对酒精诱导的小鼠肝脂质过氧化和血清ALT活性变化的影响  

Effect of tea polyphenols on liver MDA levels and serum ALT activity in alcohol-treated mice

作　　者：林春兰[1] 蒋建伟[1] 严玉霞[1] 周羽竝[1] 赵迎社[1] 吴美玉[1] 

机构地区：[1]暨南大学医学院生物化学教研室,广东广州510632

出　　处：《中国病理生理杂志》2003年第1期110-112,共3页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No.0 0 0 754)

摘　　要：目的 :研究茶多酚 (TP)对酒精性肝损伤的防治作用。方法 :通过离体和整体实验 ,采用分光光度法和血清生化检测法 ,检测肝组织脂质过氧化产物丙二醛 (MDA)水平和血清谷丙转氨酶 (ALT)的活性。结果 :离体实验中 ,TP +酒精组肝MDA水平明显低于生理盐水 +酒精组 (P <0 0 1 )。整体实验中 ,TP +酒精组小鼠肝脏MDA水平和血清中ALT活性均显著低于生理盐水 +酒精组 (P <0 0 5)。此外 ,灌酒精 1h后再给TP组 ,小鼠肝脏MDA含量也明显低于酒精 +生理盐水组 (P <0 0 1 )。结论 :茶多酚能抑制酒精引起的小鼠肝组织MDA水平和血清ALT活性的升高 。AIM: To investigate the effect of tea polyphenols (TP) on alcohol-induced liver injury in mice.METHODS: 40% alcohol(0 5 mL) and TP (5 mg) were administered intragastrically, the liver MDA level and serum alanine transaminase (ALT) activity were determined. The effect of TP on MDA level in alcohol-treated liver in vitro was also examined.RESULTS: Pretreatment with TP significantly inhibited alcohol-induced liver MDA increase in mice in vivo and in vitro , the increase in serum ALT induced by alcohol was also reduced by pretreatment with TP (0 5 mg). TP at a dose of 5 mg, administered 1 h after alcohol treatment, also suppressed increase in liver MDA level stimulated by alcohol. CONCLUSION: These results demonstrated that TP has a protective effect on alcohol-induced liver injury in mice.

关 键 词：茶多酚 酒精诱导 小鼠 肝脂质过氧化 血清 ALT 肝损伤 

分 类 号：R575[医药卫生—消化系统]