胃癌患者胃液中特异性荧光物质的初步确定  

Preliminary identification of a specific fluorescent substance in gastric juice from patients with gastric carcinoma

作　　者：李渊[1] 林三仁[1] 周丽雅[1] 彭嘉柔[1] 郭慧兰[2] 

机构地区：[1]北京大学第三临床医院消化科,北京100083 [2]北京大学医学部医药卫生分析中心

出　　处：《中华消化杂志》2003年第1期15-18,共4页Chinese Journal of Digestion

基　　金：2 0 0 1年教育部科学技术研究重点项目资助 ( 0 10 0 7)

摘　　要：目的　从胃癌患者胃液中发现特异性荧光物质 ,并以此来获得肿瘤标志物。方法　收集10例胃癌患者与 10例非胃癌患者的胃液 ,浓缩后行高效液相分析 ,并收集高效液相荧光光谱中保留时间为 11.5～ 12 .5min的荧光物质 ;再经质谱鉴定 ,所得结果与L 色氨酸的质谱比较 ,最终确定这种荧光物质的主要组分。结果　在高效液相的荧光光谱保留时间为 11.5～ 12 .5min时 ,胃癌患者胃液中有特异性荧光物质 ,其含量较其他胃内良性病变患者明显增多。经质谱鉴定后显示 ,胃癌患者胃液中质荷比为 2 0 5的物质与L 色氨酸的三级质谱完全一致 ,证实该物质为L 色氨酸。结论　L 色氨酸是胃癌患者胃液中特异性荧光物质的主要组分。Objective To investigate some specific fluorescent substances in gastric juice from patients with gastric carcinoma and to establish biomarkers for the detection of gastric cancer. Methods We collected gastric juice from 10 patients with gastric carcinoma and 10 patients without gastric carcinoma respectively. After being condensed, the specimens of gastric juice were analyzed by means of high performance liquid chromatography (HPLC). We collected the specific fluorescent substance in the retention time of 11.5-12.5 min in fluorescence spectra of HPLC and then detected them through mass spectrometry. Comparing such spectra with those of L-tryptophan, we attempted to find the principle component of the fluorescent substances. Results There was a specific substance in gastric juice in the retention time of 11.5-12.5 min in fluorescent spectra of HPLC, which was more abundant in gastric malignancy than in gastric benigh diseases. With the mass charge ratio(m/z)of 205, the substance had the same mass spectrum as shown in the third level of L-tryptophan, which was confirmed by mass spectrometry. This substance was identified to be L-tryptophan. Conclusions L-tryptophan is one of the specific fluorescent substances in gastric juice from patients with gastric carcinoma.

关 键 词：特异性荧光物质 胃癌 高效液相 质谱 

分 类 号：R735.2[医药卫生—肿瘤]