大鼠发育过程中海马CA1区nNOS的表达  

nNOS expression in developing hippocampal CA1 of rats

作　　者：何玲[1] 窦肇华[1] 王强[2] 刘晓东[1] 鲁志博[1] 

机构地区：[1]第四军医大学吉林军医学院组织胚胎学教研室,吉林吉林132013 [2]第四军医大学吉林军医学院附属医院泌尿外科,吉林吉林132013

出　　处：《第四军医大学学报》2003年第1期35-36,共2页Journal of the Fourth Military Medical University

摘　　要：目的 :研究出生后不同发育阶段大鼠海马CA1区nNOS的表达及形态学变化 ,分析nNOS的表达与海马神经元的发育、突触的形成及海马学习记忆功能的关系 .方法 :用免疫组织化学方法和图像分析对生后 1,2 ,3,4wk和 3mo龄及老龄 (2 4～ 2 8mo)大鼠海马CA1区nNOS的表达进行定位和定量分析 ,并进行光、电镜形态学检查 .结果 :大鼠海马CA1区nNOS免疫阳性细胞面数密度 :2wk (36 .2± 4 .2 )mm-2 和 3wk (33.1± 4 .1)mm-2 组高于 1wk (2 4 .9± 4 .8)mm-2 ,4wk(2 4 .6± 5 .9)mm-2 ,3mo (2 5 .4± 5 .6 )mm-2 和老龄组 (2 4 .3± 8.3)mm-2 (P <0 .0 1) ,而前两组之间及后 4组之间均无显著差异 (P >0 .0 5 ) .nNOS免疫阳性细胞积分吸光度值 :以 2wk(8.5± 2 .2 )和 3wk (8.1± 1.9)组为最高 ,其次为 4wk (4.9± 0 .5 )和 3mo (5 .2± 0 .9)组 ,1wk(3.3±0 .8)和老龄组 (3.2± 0 .7)最低 (P <0 .0 1) ;2wk和 3wk组之间、4wk与 3mo组之间、1wk和老龄组之间均无显著差异(P >0 .0 5 ) .结论 :NO与海马的发育、突触的形成和成熟以及学习记忆具有密切关系 .AIM: To investigate the expression of nNOS and the morphology change in developing hippocampal CA1 of rats and analyse the relation of the expression of nNOS to the development of hippocapus's neurons,formation of synapse and fanction of learning memory. METHODS: By means of immunohistochemistry and image analysis, we investigate the expression of nNOS in developing hippocampus (CA1) of rats aged from 1 to 4 wk and 3 mo and 24～28 mo.We also made a morphological examination of them with optical and electric microscope. RESULTS: The numerical densities on area of nNOS positive cells of 2 wk (36.2 ±4.2) mm -2 and 3 wk(33.1±4.1)mm -2 groups were higher than those of 1 wk(24.9±4.8)mm -2 , 4 wk(24.6± 5.9) mm -2 , 3 mo(25.4±5.6)mm -2 and 24～28 mo(24.3± 8.3) mm -2 ( P <0.01). The differences between the former two groups and among the latter four groups were not significant ( P >0.05). Comparison of the IOD of positive cells: From high to low was 2 wk(8.5±2.2) and 3 wk(8.1±1.9), 4 wk(4.9±0.5) and 3 mo(5.2±0.9), 1 wk(3.3±0.8) and aged group(3.2±0.7) by turn, with that of the last group being the lowest ( P <0.01). There were no significant differences between 2 and 3 wk, 4 wk and 3 mo, 1 wk and aged group ( P >0.05). CONCLUSION: With change of age, the expression of nNOS in hippocampus (CA1) appears to have an obvious development change trend.

关 键 词：大鼠 海马 CA1区 NNOS 免疫组织化学 

分 类 号：R338.26[医药卫生—人体生理学]