拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导  

Polyphenol Oxidase, Peroxidase and Phenylalanine Ammonium Lyase in Postharvest Peach Fruits Induced by Inoculation withPichia membranefaciens or Rhizopus stolonifer

作　　者：秦国政[1] 田世平[1] 刘海波[1] 徐勇[1] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093

出　　处：《中国农业科学》2003年第1期89-93,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 0 170 663 ) ;中国科学院植物研究所"知识创新"前沿资助项目

摘　　要：Pichiamembranefaciens能有效抑制桃果实采后软腐病。笔者观测和比较接种拮抗酵母菌P .mem branefaciens和软腐病菌Rhizopusstolonifer对采后桃果实多酚氧化酶 (PPO)、过氧化物酶 (POD)和苯丙氨酸解氨酶(PAL)活性的诱导情况。试验结果表明 ,桃果实接种P .membranefaciens +R .stolonifer 2 4h后 ,PPO和PAL活性开始升高 ,并在整个试验过程中一直保持较高的水平。只接种R .stolonifer也能诱导桃果实PPO和PAL活性的增加 ,但效果不如接种拮抗菌 +病原菌的好。然而 ,接种拮抗菌 +病原菌和只接种病原菌对诱导POD活性都没有明显的作用。可以认为 。Rhizopus rot of peaches could be significantly suppressed by Pichia membranefaciens. Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium lyase (PAL) activities induced by inoculation with P. membranefaciens and R. stolonifer were studied in postharvest peach fruits. The activities of PPO and PAL in peaches increased significantly after 24 h of inoculation with P. membranefaciens + R. stolonifer, and maintained at a high level throughout the experiment. PPO and PAL could also be triggered by infection with R. stolonifer alone, but the levels of activity were lower than those treated with P. membranefaciens + R. stolonifer. However, fruits inoculated with P. membranefaciens + R. stolonifer or R. stolonifer alone did not stimulated POD activity. The results suggest that the activation of these defense enzymes is involved in the action of P. membranefaciens against R. stolonifer.

关 键 词：拮抗菌 病原菌 桃果实 多酚氧化酶 过氧化物酶 苯丙氨酸解氨酶 抗性相关酶 酶活性 抑菌作用 软腐病 

分 类 号：S662.109[农业科学—果树学]