荧光原位杂交检测卵巢癌8号染色体畸变  

Detection of chromosome 8 anomalies in ovarian carcinoma by fluorescence in situ hybridization

作　　者：董跃兰[1] 李春华[2] 陈利馨[1] 冯怀英[2] 朱宝生[2] 

机构地区：[1]云南省第一人民医院妇科,昆明650032 [2]云南省第一人民医院计划生育科,昆明650032

出　　处：《中华医学遗传学杂志》2003年第1期59-60,共2页Chinese Journal of Medical Genetics

基　　金：云南省应用基础研究基金 (97C0 73M)~~

摘　　要：目的　探讨卵巢癌染色体改变与卵巢癌发生、发展及预后的关系。方法　应用荧光原位杂交技术对卵巢癌、卵巢良性肿瘤及正常卵巢标本各 12例进行 8号染色体检测。结果　本组 12例卵巢癌 8号染色体出现单体、三体或四体畸变 ,畸变率 10 0 % (12 /12 ) ;卵巢良性肿瘤 8号染色体出现三体畸变 ,畸变率2 5 % (3/12 ) ;正常卵巢 8号染色体无畸变 ,卵巢癌与卵巢良性肿瘤及正常卵巢比较 ,差异有显著性 (P<0 .0 0 1)。结论　卵巢癌第 8号染色体畸变与卵巢癌发生、发展密切相关 ,其改变发生在卵巢癌早期 ,与患者临床分期。Objective: To detect the relationship between chromosomal anomalies and the pathogenesis, development and prognosis of ovarian carcinoma. Methods Thirty-six specimens of ovarian carcinoma (n = 12), ovarian benign tumor (n = 12), and normal ovary (n = 12) were examined by fluorescence in situ hybridization (FISH). Results: Twelve cases of mutations, including trisomy 8, monosomy 8 or tetraploid 8 chromosomal anomalies, were found in the group of ovarian carcinoma, making up 100% (12/12). Three cases of trisomy 8 chromosomal anomalies were found in the group of ovarian benign tumor, accounting for 25% (3/12). No anomaly was found in the normal group. There were significant differences between the three groups, P<0.001. Conclusion: The above anomalies of chromosome 8 are significantly associated with the pathogenesis and development of ovarian carcinoma. The anomalies may occur in the early stage of the carcinoma, and may be significantly associated with the pathological differentiation and clinical stage of the case.

关 键 词：荧光原位杂交 检测 卵巢癌 染色体畸变 

分 类 号：R737.31[医药卫生—肿瘤]