核仁组成区嗜银蛋白和增殖指数在肾母细胞瘤研究中的应用  

Use of AgNOR and Flow Cytometry in Study of Wilms′Tumor

作　　者：周晓岩[1] 王琳[2] 姜云鹏[2] 陈远耀[2] 

机构地区：[1]吉林大学第一医院儿外科,吉林长春130021 [2]吉林大学基础医学院病理学教研室

出　　处：《吉林大学学报（医学版）》2003年第1期79-81,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科委应用基础研究资助课题 (92 6 0 31)

摘　　要：目的 :研究肾母细胞瘤的组织学分型、临床分期与 DNA增殖指数及核仁组成区相关嗜银蛋白 ( Ag NOR)间的关系。方法 :通过流式细胞仪测定增殖指数 ( proliferating index,PI) ;用组织化学法检测 Ag NOR。结果 :正常肾脏与肾母细胞瘤组织间的 Ag NOR颗粒计数、颗粒直径及形状因子均有明显差异 ;间变型的 Ag NOR颗粒计数 ( 4 .2 )显著高于无间变型 ( 3.2 8) ;Ag NOR颗粒计数随分期增高而增高 ;高 Ag NOR计数组的 PI值 ( 4 4.35 )显著高于低 Ag NOR计数组 ( 32 .5 5 )。结论 :PI值及 AgObjective: The present study attempts to investigate the possible association between histological types, proliferative index and argyrophilic nucleolar organizer regions(AgNOR) in Wilms′ tumor. Methods: Proliferative index (PI) was assessed by flow cytometry(FCM); AgNOR was evaluated by histochemistry. Results: A good differentiation between normal kideny and Wilm′s tumor was obtained by the counts, diameter and shape factor of AgNOR granulars; AgNOR counts were higher in anaplasia type than in non anaplasia type(4.20 vs . 3.28); A significant correlation could be found between AgNOR and stages; PI of the patients with higher AgNOR score was significantly higher than that of patients with low AgNOR score( 44.35 vs . 32.55). Conclusion: PI and AgNOR might be very useful prognostic factors in patients with Wilms′tumor.

关 键 词：肾母细胞瘤 流式细胞术 核仁组成区嗜银蛋白 

分 类 号：R737.11[医药卫生—肿瘤]