Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin  

作　　者：YOGESHWER SHUKLA 1Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Post Box 80, M. G. Marg, Lucknow-226 001, India 

出　　处：《Biomedical and Environmental Sciences》2001年第3期237-240,共4页生物医学与环境科学（英文版）

摘　　要：Objective Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated. Methods The fresh frozen tissue sections (8m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange. Results The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin. Conclusion The activity of the NBT-reductase seems to be dependent on the glutathione contents.Objective Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated. Methods The fresh frozen tissue sections (8m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange. Results The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin. Conclusion The activity of the NBT-reductase seems to be dependent on the glutathione contents.

关 键 词：ANIMALS Cell Division FEMALE GLUTATHIONE Mice Nitroblue Tetrazolium OXIDOREDUCTASES Skin 

分 类 号：Q593[生物学—生物化学]