Erianin induces apoptosis in human leukemia HL-60 cells  

毛兰素诱导人白血病HL-60细胞的凋亡(英文)

作　　者：李运曼[1] 王海燕[2] 刘国卿 

机构地区：[1]中国药科大学药理教研室 [2]Shengli Oilfield Shengli Hospital,Dongying 257055,China

出　　处：《Acta Pharmacologica Sinica》2001年第11期1018-1022,共5页中国药理学报（英文版）

摘　　要：AIM: To investigate the effect of erianin on human HL-60 cell line and explore its mechanism of apoptosis in vitro. METHODS: Inhibition of proliferation was measured with colorimetric MTT assay. The morphologic changes were observed by fluorescence and electron microscopes. DNA fragmentation was visualized by agarose gel electrophoresis, and the DNA degradation was determined by flow cytometry. Immunohistochemical analysis was used to identify the expression of bcl-2 and bax genes. RESULTS: The growth of human HL-60 cells was significantly inhibited by erianin 20 - 81.9 nmol/L during 72 h treatment ( P < 0.01). The IC50 value was 38 nmol/L after a 24-h exposure to erianin, while that of vincristine, the positive control, was 101 nmol/L. The typical morphologic changes were observed and the nuclear DNA fragmentation exhibited 'ladder' pattern. The cell cycle of HL-60 cells was arrested in G2/M phase, and expression of bcl-2 gene was decreased while that of bax was increased. CONCLUSION: Erianin showed potent inhibitory activity on the proliferation of HL-60 cells. The inhibition might be relative to the apoptosis induced by erianin and the altered expression of bcl-2 and bax genes in HL-60 cells.目的:研究毛兰素对HL-60细胞增殖的抑制作用,探讨其诱导细胞凋亡的机制。方法:用MTT比色法测定了毛兰素对HL-60细胞增殖的抑制作用:应用荧光显微镜、透射电镜、DNA电泳及流式细胞仪观察了药物对细胞凋亡的诱导作用,并用免疫组化的方法从基因水平阐述了凋亡的发生。结果:毛兰素20-81.9nmol/L在72h内显著抑制HL-60细胞增殖,作用24h后,对HL-60细胞的IC_(50)为38nmol/L,而阳性对照药长春新碱对HL-60细胞的IC_(50)为101nmol/L,前者明显优于后者;形态学观察可见凋亡的特征性改变;琼脂糖电泳出现典型的DNA“ladder”;流式细胞仪结果表明细胞被阻滞于G_2/M期;免疫组化可见bcl-2表达下降,bax表达升高。结论:毛兰素显著抑制HL-60细胞的生长,该抑制作用可能是通过诱导细胞凋亡和改变HL-60细胞bcl-2和bax基因的表达而实现的。

关 键 词：ERIANIN LEUKEMIA HL-60 cells APOPTOSIS DNA fragmentation cell cycle bcl-2 genes 

分 类 号：R733.7[医药卫生—肿瘤]