乙型肝炎病毒x基因转基因小鼠的表型分析  

Phenotype analysis of transgenic mice harbouring hepatitis B virus x gene

作　　者：熊俊[1] 訾晓渊[1] 姚玉成[1] 金艳花[1] 李建秀[1] 苏小平[1] 王新民[1] 倪文君[1] 丛文铭[2] 胡以平[1] 

机构地区：[1]第二军医大学基础医学部细胞生物学教研室,上海200433 [2]第二军医大学东方肝胆外科医院病理科

出　　处：《第二军医大学学报》2003年第2期155-158,共4页Academic Journal of Second Military Medical University

基　　金：国家"九五"攻关项目 (TJ99-LA0 1) ;国家自然科学基金 (3 9670 811) ;上海市科学技术发展基金项目 (9949190 3 3 )

摘　　要：目的 :研究乙型肝炎病毒 X蛋白致转基因小鼠的表型效应。 方法 :采用免疫组织化学和组织病理学方法分析转基因小鼠中 X蛋白的表达部位及其病理学改变。结果 :免疫组织化学检测发现 X蛋白在转基因小鼠的肝脏、脾脏、肺脏、肾脏和皮肤组织中均有表达 ,从 F0 代到 F3代小鼠 ,X蛋白在肝细胞中的分布逐渐从细胞质向细胞核转移。病理学分析显示 ,表达 X蛋白的转基因小鼠出现了皮肤组织结构异常、肝组织和肺组织轻度变性等病理学变化。 结论 :乙型肝炎病毒 x基因转基因小鼠的肝脏、脾脏、肺脏、肾脏和皮肤等组织中表达 X蛋白 ,部分转基因小鼠出现的皮肤组织、肝组织和肺组织病理性损伤可能与 X蛋白有关 ;各代转基因小鼠肝细胞中 X蛋白的分布具有规律性的变化 ,可用于研究Objective:To study the phenotype induced by hepatitis B virus X protein in transgenic mice. Methods:Immunohistochemistry and pathological methods were used to analyze the subcellular localization of X protein and pathological change in transgenic mice.Results:Expression of X protein was detected in liver,spleen,lung,kidney and skin of transgenic mice by immunohistochemistry. From F 0 to F 3 offsprings,the subcellular localization of X protein was changed from hepatocytes cytoplasm to nuclear. Pathological findings showed that transgenic mice expressing X protein had some pathological changes,including abnormal change of skin tissue and degeneration of liver and lung tissue. Conclusion: X protein can be expressed in liver,spleen,lung,kidney and skin of the hepatitis B virus x gene( HBx ) transgenic mice. It may be a pathogenic factor inducing pathological lesions in skin tissue,liver and lung of HBx transgenic mice. The change of subcellular localization of X protein in hepatocytes of transgenic mice is useful for analyzing the involvement of X protein in signal transduction pathways.

关 键 词：乙型肝炎病毒 X基因 转基因小鼠 病理学 免疫组织化学 

分 类 号：R373.21[医药卫生—病原生物学]