细胞膜磷脂组分含量变化与结直肠癌生物学行为的关系研究  

Study on the relationship of membrane phospholipid variations with biological behavior of colorectal carcinoma

作　　者：安萍[1] 于波[1] 李世拥[1] 张英男[1] 魏家臣[1] 蔡慧芸[1] 

机构地区：[1]北京军区总医院普通外科,100700

出　　处：《中华胃肠外科杂志》2003年第2期117-119,共3页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金资助项目(30070747)

摘　　要：目的探讨结直肠癌细胞膜磷脂变化对结直肠癌生物学行为的影响。方法检测48例癌原发灶、癌旁肠黏膜、肝转移灶中细胞膜磷脂酰肌醇(phosphatidylinosital,PI)、磷脂酰丝氨酸(phosphatidylserine,PS)、磷脂酰乙醇胺(phosphatidylethanolamine,PE)和磷脂酰胆碱(phosphatidylc-holine,PC),对比分析不同膜磷脂组分含量变化与癌原发灶大小、病理组织学类型和微血管密度的关系。结果48例结直肠癌患者癌旁肠黏膜、原发灶、肝转移灶中细胞膜磷脂PI含量分别为(0.92±0.12)mg/g、(1.57±0.14)mg/g、(1.54±0.15)mg/g;PC含量分别为:(56.47±5.33)mg/g、(108.57±6.37)mg/g、(116.35±6.85)mg/g。原发灶和肝转移灶中PI、PC含量明显高于癌旁肠黏膜组织(F=363.10、870.10,P<0.01)。3种组织中PE含量分别为(18.23±3.56)mg/g、(42.02±4.33)mg/g、(79.51±5.52)mg/g,肝转移灶中PE含量显著高于原发灶和癌旁组织(F=1149.63,P<0.01)。不同大小的癌原发灶4种膜磷脂组分含量差异无显著性意义(F=0.011、0.026、0.305、1.483;P>0.05)。结论细胞膜磷脂变化对结直肠癌细胞的生物学行为可能有重要影响。PI、PC变化与结直肠癌的发生有关,PE含量升高与结直肠癌肝转移有密切关系。Objective To investigate the contents of membrane phospholipid variations and their relationship with biological behavior of colorectal carcinoma. Methods High performance liquid chromatography was used to detect the contents of membrane phospholipids such as phosphatidylinosita (PI), phosphatidylidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in primary foci, para tumor mucosa and hepatic metastases of 48 cases with colorectal carcinoma. The relationship of their contents with the lesion size,pathological type and microvessel density were analyzed. Results PI content was (0 92±0 12)mg/g,(1 57±0 14)mg/g,(1 54±0 15)mg/g respectively, and PC content was(56 47±5 33)mg/g,(108 57±6 37)mg/g,(116 35±6 85)mg/g in primary foci, para tumor mucosa and hepatic metastases. PI and PC contents in primary foci and hepatic metastases were significantly higher than those in para tumor mucosas(F=363 10、F=870 10,P< 0 01). PE content was (18 23±3 56)mg/g,(42 02±4 33)mg/g,(79 51±5 52)mg/g in three kinds of tissues,and the highest in hepatic metastases(F=1149 63,P< 0 01). No significant difference was found in the contents of membrane phospholipids between the cases with different lesion sizes (F=0 011、0 026、0 305、1 483;P >0 05). Microvessel density in primary foci with hepatic metastasis was greater than that without metastasis. Conclusions Membrane phospholipid variations may have crucial effects on the biological behavior of colorectal cancer. Increasing PI and PC contents may be related with carcinogenesis, and high PE with hepatic metastasis.

关 键 词：结肠直肠肿瘤 细胞膜 磷脂类 生物学 

分 类 号：R735.3[医药卫生—肿瘤]