共轭亚油酸对人胃腺癌细胞侵袭及转移相关基因表达的影响  

The effects of conjugated linoleic acid on the expression of invasiveness and metastasis-associated gene of human gastric carcinoma cell line

作　　者：杨艳梅[1] 陈炳卿[1] 郑玉梅[1] 王璇琳[1] 刘家仁[1] 薛英本[1] 刘瑞海 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,150001 [2]Cornell University,Ithaca

出　　处：《中华预防医学杂志》2003年第1期26-28,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助 ( 30 0 70 6 58)

摘　　要：目的　研究c9,t1 1 共轭亚油酸 (CLA)对人胃腺癌细胞 (SGC 790 1 )侵袭能力的影响 ,探讨其抑制肿瘤转移的可能机制。方法　用重组基底膜侵袭实验评价癌细胞侵袭能力 ;用逆转录聚合酶链反应 (RT PCR)检测SGC 790 1细胞中组织基质金属蛋白酶抑制剂 (TIMP) 1、TIMP 2和nm2 3 H1mRNA的表达。结果　在 2 0 0、1 0 0和 50 μmol/L浓度时 ,c9,t1 1 CLA对SGC 790 1细胞侵袭重组基底膜的抑制率分别为 53 7%、40 9%和 2 9 3 %。c9,t1 1 CLA可诱导SGC 790 1细胞中TIMP 1、TIMP 2和nm2 3 H1 mRNA的表达。结论　c9,t1 1 CLA抑制SGC 790 1细胞侵袭重组基底膜。c9,t1 1 CLA的抗侵袭活性与诱导肿瘤细胞中TIMP 1、TIMP 2和nm2 3 H1Objectives To study the effects of c9,t11 conjugated linoleic acid (c9,t11 CLA) on invasive ability of human gastric carcinoma cell line (SGC 7901) and to explore its possible mechanism Methods Reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells Expression of TIMP 1,TIMP 2 and nm23 H 1 mRNA was measured by reverse transcription polymerase chain reaction (RT PCR) in SGC 7901 cells Results At the concentrations of 200 μmol/L,100 μmol/L and 50 μmol/L,c9,t11 CLA suppressed their reconstituted basement membrane invasion of SGC 7901 by 53 7%,40 9% and 29 3%,respectively c9,t11 CLA could induce the expression of TIMP 1,TIMP 2 and nm23 H 1 mRNA in SGC 7901 cells Conclusions The invasion of SGC 7901 cells could be inhibited by c9,t11 CLA through reconstituted basement membrane Anti invasion action of c9,t11 CLA might be associated with induction of expression of TIMP 1, TIMP 2 and nm23 H 1 mRNA in tumor cells

关 键 词：胃肿瘤 腺癌 共轭亚油酸 CLA 肿瘤转移 

分 类 号：R735.2[医药卫生—肿瘤]