Alzheimer病ApoE基因多态性研究  

Study of ApoE Gene Polymorphism of Alzheimer Disease

作　　者：李栋[1] 刘元华[2] 董选[2] 高锦声[1] 

机构地区：[1]苏州大学生命科学学院,苏州215007 [2]苏州大学附属第三医院,常州213003

出　　处：《苏州大学学报（医学版）》2003年第1期30-32,共3页Suzhou University Journal of Medical Science

摘　　要：目的　探讨Alzheimer病 (AD)与载脂蛋白E(ApoE)基因多态性的关系。 方法　应用快速PCR方法与荧光能量共振转移 (FRET)相结合对 2 5例AD患者及 5 9名健康老人的ApoE基因进行检测 ,对AD患者的ApoE基因多态性进行相关分析。结果　两组人群ε3/ε3基因型比例最大 ,未发现ε2 /ε2 基因型。AD组、对照组ε2 、ε3、ε4 3种等位基因的频率 (% )分别为 10、62、2 8;7.63、78.81、13 .5 6。ApoEε4 基因的频率AD组 (2 8% )明显高于对照组(13 .5 6% ) (P <0 .0 5 )。 2 5例AD患者中有 12例 (48% )ε4 基因携带者 ,而 5 9例对照组有 13例 (2 2 % )。ApoEε4 基因与AD的发生有显著相关性 (P <0 .0 5 ) ,其危险系数RR =2 .2。结论　ε4 等位基因与痴呆发病有一定关联 。Objective To study the relationship between the apolipoprotein E(ApoE) gene polymorphism and Alzheimer disease(AD).Methods The polymorphism of the ApoE gene was detected using rapid-cycle polymerase chain reaction(PCR) and fluorescence resonance energy transfer(FRET) technique for 25 patients with AD and the controls.Results The most frequent genotype was ε 3/ε 3,but ε 2/ε 2 was not observed in the 2 groups.The estimated ApoE allele frequencies (%) in AD group were 10、62、28 for the ε 2、ε 3、ε 4 alleles;and 7.63、78.81、13.56 in the control group.The frequencies of the ApoE ε 4 in AD group (28%) were significantly higher than those in the controls (13.56%) ( P <0.05).There were 12 ApoE ε 4 carriers in AD group of 25 and 13 carriers in control group of 59. ApoE ε 4 has significant co relation to the occurrence of AD ( P <0.05),RR=2.2.Conclusions ApoE ε 4 allele has definite co relation to the occurrence of AD,it is a risk facter for the occurrence of AD.

关 键 词：ALZHEIMER病 APOE 等位基因 

分 类 号：R749.16[医药卫生—神经病学与精神病学]