共轭亚油酸对人胃腺癌细胞侵袭能力的影响及其作用机制  

Effects of c9,t11-conjugated linoleic acid on the metastasis of human gastric carcinoma cell line

作　　者：杨艳梅[1] 陈炳卿[1] 薛英本[1] 郑玉梅[1] 王璇琳[1] 刘家仁[1] 刘瑞海 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150001 [2]CornellUniversity

出　　处：《卫生研究》2003年第2期117-119,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 0 70 658)

摘　　要：研究c9,t11-共轭亚油酸 (CLA)对人胃腺癌细胞 (SGC 790 1)侵袭能力的影响 ,探讨其抑制肿瘤转移的可能机理。用 0、2 5、5 0、10 0和 2 0 0 μmol LCLA处理细胞 2 4h后 ,分别用重组基底膜侵袭实验评价癌细胞侵袭能力 ;PAGE底物酶谱方法检测Ⅳ型胶原酶活性 ;RT PCR方法检测SGC 790 1细胞TIMP 1和TIMP 2mR NA表达。研究结果表明 :c9,t11 CLA处理后 ,SGC 790 1细胞侵袭重组基底膜的能力下降、SGC 790 1细胞培养上清中的Ⅳ型胶原酶活性降低、SGC 790 1细胞中TIMP 1和TIMP 2mRNA的表达增加。因此 ,c9,t11 CLA抑制SGC 790 1细胞侵袭重组基底膜 ,并且c9,t11 CLA的抗侵袭活性与降低肿瘤细胞培养上清中Ⅳ型胶原酶活性和诱导肿瘤细胞TIMP 1和TIMPIn order to study the effects of c9,t11 conjugated linoleic acid on the invasion of human gastric carcinoma cell line(SGC 7901) and explore its possible mechanism, the five levels of CLA in medium were designed as 0, 25, 50,100 and 200 mmol/L respectively. After SGC 7901 were treated with c9,t11 CLA for 24 hours, the reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. The type Ⅳ collagenase was assessed by PAGE substrate zymography and the expression of TIMP 1 and TIMP 2 mRNA was measured by RT PCR. The result displayed that c9,t11 CLA could inhibit the reconstituted basement memebrane invasion,reduce type Ⅳ collagenase activities in the serum free supernatant of SGC 7901 cells and induce TIMP 1 and TIMP 2 mRNA expression in SGC 7901 cell line. Therefore, it is suggested that CLA could inhibit the reconstituted basement membrane invasion of SGC 7901 cells and the anti invasion action of c9,t11 CLA might be associated with the reduction of type Ⅳ collagenase activity in tumor cell culture supernatant and induction of tumor cell TIMP 1 and TIMP 2 mRNA expression.

关 键 词：共轭亚油酸 人胃腺癌细胞 SGC-7901 胶原酶 TIMP-1 TIMP-2 

分 类 号：R735.2[医药卫生—肿瘤] R730.1[医药卫生—临床医学]