水杨酸与烟草铜锌超氧化物歧化酶的相互作用  

The Interaction of CuZn-superoxide Dismutase and Salicylic Acid

作　　者：盛良全[1] 刘少民[2] 贾莉莉[2] 徐小龙[1] 解永树[1] 刘清亮[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026 [2]中国科学技术大学食品系,合肥230052

出　　处：《分析化学》2003年第3期283-286,共4页Chinese Journal of Analytical Chemistry

摘　　要：利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸 (SA)对超氧化物歧化酶 (SOD)活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制 ,求得两者结合的形成常数和配位数 ,使用F rster非辐射能量转移技术求出了两者的结合位置距色氨酸残基间的距离为 2 .60nm。The interaction of CuZn superoxide dismudase (SOD) and salicylic acid (SA) by the enzyme activity was studied. Meanwhile, the static fluorescence quenching mechanism revealed that the complex of CuZnSOD with salicylic acid was formed. The binding constant was obtained from lineweaver-burk and double-lg plot. The distance of active site was about 2.60 nm calculated according to Forster theory.

关 键 词：水杨酸 铜锌超氧化物歧化酶 酶活力 荧光光谱 烟草 相互作用 邻苯三酚自氧化法 

分 类 号：O657.3[理学—分析化学]