Effects of propofol, midazolam and thiopental sodium on outcome and a mino acids accumulation in focal cerebral ischemia-reperfusion in rats  

作　　者：陈莲华 贡沁燕 肖常思 

机构地区：[1]Department of Anesthesiology,The Ophlhalmology and Otorhinlaryngology Hospital,Fudan University [2]Department of Pharmacology,School of Basic Medical Sciences,Fudan University

出　　处：《Chinese Medical Journal》2003年第2期292-296,共5页中华医学杂志（英文版）

摘　　要：To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia reperfusion in rats Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3 hour middle cerebral artery occlusion by intraluminal suture and 24 hour reperfusion Neurologic outcomes were score d on a 0-5 grading scale Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol inc reased gama aminobutyric acid accumulation in ischemic areas in reperfusion Conclusion Propofol and midazolam, but not thiopental sodium, may provide protective effect s against reperfusion induced injury in rats subjected to focal cerebral ischemi a This neurological protection may be due to the acceleration of excitatory am ino acids elimination in reperfusionTo investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia reperfusion in rats Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3 hour middle cerebral artery occlusion by intraluminal suture and 24 hour reperfusion Neurologic outcomes were score d on a 0-5 grading scale Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol inc reased gama aminobutyric acid accumulation in ischemic areas in reperfusion Conclusion Propofol and midazolam, but not thiopental sodium, may provide protective effect s against reperfusion induced injury in rats subjected to focal cerebral ischemi a This neurological protection may be due to the acceleration of excitatory am ino acids elimination in reperfusion

关 键 词：cerebral ischemia REPERFUSION amino acids PROPOFOL MIDAZOLAM thiopental sodium 

分 类 号：R614[医药卫生—麻醉学]