雪旺细胞源性神经细胞黏附分子L_1对脊髓轴突再生作用的研究  

Effect of Schwann cell-derived neural cell adhesion molecule L_1 on axon regeneration of injured spinal cord

作　　者：魏海峰[1] 曹莉[2] 肖建如[1] 贾连顺[1] 袁文[1] 石学银[3] 叶晓健[1] 桂斌捷[1] 何成[2] 

机构地区：[1]上海长征医院骨科,200003 [2]第二军医大学神经生物教研室 [3]上海长征医院麻醉科,200003

出　　处：《中华骨科杂志》2003年第3期165-169,共5页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金资助项目(39870759)

摘　　要：目的研究神经细胞黏附分子L1抗体封闭后的雪旺细胞对脊髓损伤后轴突再生的影响。方法将纯度高于98%的新生SD大鼠坐骨神经源性雪旺细胞、经神经细胞黏附分子L1抗体封闭后的雪旺细胞及生理盐水分别移植于成年SD大鼠T10节段左侧胸髓半横切损伤处(各组大鼠均为24只),8周后采用辣根过氧化物酶(HRP)逆行示踪、NF免疫组化染色及Westernblot蛋白定量等方法,观察损伤脊髓的神经轴突再生情况。结果L1抗体封闭组与应用未封闭雪旺细胞组相比,HRP阳性神经元数目明显减少,再生神经轴突量减少,Westernblot显示前者NF量仅为后者的2/3左右。结论雪旺细胞源性神经细胞黏附分子L1对损伤脊髓的轴突再生具有促进作用。Objective To study the effect of Schwann cell enclosed by antibody of neural cell adhesion molecule L1 on injured spinal cord. Methods Over 98% of the purity of Schwann cells obtained from bilateral sciatic nerves of 2 days newborn SD rats, the concentration of Schwann cells was about 2.5×104 /μl. The Schwann cells were enclosed by the antibody of neural cell adhesion molecule L1 using co-culture. The adult SD rats (weight 200-250 g) were used to establish the model of spinal cord injury by hemi-transection at the left side of T10 level. The animals were divided into three groups; the SC group was transplanted with 20 μl suspension Schwann cells; the anti-L1 group with 20 μl Schwann cells enclosed by antibody of neural cell adhesion molecule L1; and the control group was injected solely with normal saline to the injured cord. Eight weeks later regenerated neural axons were investigated through horseradish peroxiase HRP retrograde trace immunohistochemistry of neurofilament and Western blot. Results Few regenerated neural axons appeared in the control group; some of regenerated neural axons could be observed in anti-L1 group; plentiful and bulky regenerated neural axons were found in SC group. The group with antibody had significant less HRP positive neurons and neural axons than the group without antibody. Western blot showed that the quantity of neurofilament in the anti-L1 group was only two thirds of the SC group. Conclusion Schwann cell-derived neural cell adhesion molecule L1 is able to enhance the neural axon regeneration of injured spinal cord.

关 键 词：NCAM 许旺氏细胞 神经再生 脊髓损伤 神经细胞黏附分子L1 

分 类 号：R651.2[医药卫生—外科学]