皖南地区汉族人群GSTM1基因频率及其与肺癌发生相关性的研究  

Study on the allele frequency of GSTM1 gene in normal Han population in Wan-nan area and the relationship between GSTM1 genotype and the risk of lung cancer

作　　者：陈丽君 孙惠兰 徐毓其 

机构地区：[1]皖南医学院遗传医学中心

出　　处：《皖南医学院学报》2003年第1期13-16,共4页Journal of Wannan Medical College

摘　　要：目的　研究皖南地区正常汉族人群谷胱甘肽 S转移酶M1基因的基因频率 ,分析该基因的遗传特征 ,探讨GSTM 1纯合缺失基因型与肺癌发生的相关性。方法　应用聚合酶链反应 (PCR)方法对 99例健康汉族人 ,3 8例肺癌患者及 8个家庭进行GSTM 1基因检测。结果　GSTM 1基因符合孟德尔遗传规律 ,呈常染色体显性遗传 ;皖南地区正常汉族人群组GSTM 1基因频率为 2 4.12 % ,GSTM1基因纯合缺失率为 5 7.5 % ,肺癌组GSTM 1基因纯合缺失率为 63 .2 % ,两组间差异无显著性 (χ2 =0 .3 5 ,P =0 .13 ) ;按吸烟与否分层分析表明 ,在吸烟人群中 ,肺癌患者的该基因的纯合缺失率与对照组相比亦无显著性差异 (χ2 =0 .199,P =0 .191)。Objective\ To study the allele frequency of GSTM1 gene in normal Han population in Wannan area,and to determine the pattern of inheritance of this gene and discuss the relationship between the homologous deletion of GSTM1 gene and susceptibility to lung cancer . Methods\ Polymerase chain reaction (PCR) method was used to identify the genotypes of GSTM1 gene in 8 families?38 cases with lung cancer and 99 healthy individuals. Results\ The transmission of GSTM1 gene was in accordance with Mendelian genetics with its pattern of inheritance appearing to be that of autosomal dominant; the allele frequency of GSTM1 in normal Han population of Wannan area was 24.12%,the rate of gene deletion was 57.5%,and for the cases with lung cancer the rate of gene deletion was 63.2%. There was no significant difference between the two groups statistically(χ\+2=0.35,P=0.13); an analysis stratified according to smoking and nonsmoking showed that GSTM1 gene deletion rate between the case group and control group in smokers did not differ significantly either (χ\+2=0.199,P=0.191). Conclusion\ Homozygous deletion of human GSTM1 gene may have no relation with the risk of lung cancer.

关 键 词：汉族人群 GSTM1基因 肺癌 基因频率 缺失基因 孟德尔遗传规律 皖南地区 

分 类 号：R734.2[医药卫生—肿瘤]