卵巢癌药敏试验的微量法研究  

Application of a Micromethod in Ovarian Cancer Chemosensitivity Testing in vitro

作　　者：刘珊玲[1] 钱晓蕾[1] 彭芝兰[1] 王和[1] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041

出　　处：《四川大学学报（医学版）》2003年第2期298-299,312,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　探讨微量检测 ATP在卵巢癌药敏试验中应用的可行性。方法　对 35例新鲜卵巢癌组织标本进行药敏试验 ,应用 Victor 2多功能检测仪微量检测 ATP值。结果　 1Victor 2多功能检测仪微量检测 ATP标准品与发光值的关系 ,在 ATP浓度为 10 - 9～ 10 - 5m ol/ml时 ,显示极好的线性关系 ,直线方程为 Y =0 .892 X +10 .2 5 7,相关系数 r=0 .998(P<0 .0 1)。 2微量检测 ATP的变异系数平均为 7.3%。 3微量检测 ATP进行药敏试验 ,敏感性 92 .0 % ,特异性 70 .0 % ,预测准确率为 87.1%。结论　应用 Victor 2多功能检测仪微量检测 ATP进行药敏试验 ,方法稳定 ,实验成本降低 ,更为简便、快速 ,误差小 。Objective To assess the efficacy of microdetermination in the ATP bioluminescence assay (ATP CVA) of ovarian cancer chemosensitivity testing in vitro . Methods A micromethod ATP CVA was employed in determining the chemosensitivity of 35 specimens of fresh ovarian cancer cells using Victor 2 Multilabel Counter. Results ① By use of Victor2 Mutilabel Counter to determine ATP, in a range of 10 -9 10 -5 mol/ml, a linear relationship was remarkable between the log ATP and the log luminescence. The equation was Y= 0.892X+10 257, the correlation coefficient r was 0.998, P <0.01. ② The coefficient of variation obtained from ATP CVA was 7.3%. ③ The sensitivity of the test was 92.0%, the specificity 70.0%, and the total predictive accuracy of ATP CVA 87.1%. Conclusion With the use of Victor 2 Mutilabel Counter, ATP CVA is fast, eost effective, and easy to perform with only small error.

关 键 词：药物敏感性试验 微量法 卵巢癌 ATP法 

分 类 号：R737.31[医药卫生—肿瘤] R965.3[医药卫生—临床医学]