毛细管电泳法测定玉米赤霉烯酮及其代谢物  

Determination of Zearalenone and Its Metabolites in Grains by Capillary Electrophoresis

作　　者：曾红燕[1,2] 黎源倩[1] 晋军[3] 孙惠琴[3] 

机构地区：[1]四川大学华西公共卫生学院卫生检测学教研室,成都610041 [2]华西公共卫生学院卫生检测专业2000级博士研究生 [3]四川大学分析测试中心

出　　处：《四川大学学报（医学版）》2003年第2期333-336,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金重大课题 (批准号 3 0 0 3 0 12 03 0 0 70 678)资助

摘　　要：目的　建立粮食样品中玉米赤霉烯酮及其代谢物的毛细管电泳测定方法。方法　样品经液 -液超声波提取 ,C1 8小柱净化后 ,胶束电动毛细管电泳 (MEKC)测定。结果　玉米赤霉烯酮 (Zeralenone,ZON)、α- Zearalenol(α- ZOL)、β- Zearalenol(β- ZOL)、黄曲霉 B1 (Aflatoxin B1,AFT B1 )、赭曲霉 A(Ochratoxin A,Och A)的检出限分别为 :0 .0 0 84、0 .0 81、0 .14、0 .0 0 16、0 .0 31μg/m l。样品加标回收率 77.9%～ 10 3.1% ,相对标准偏差 0 .6 3%～ 1.98%。结论　该法灵敏、准确 。Objective To establish a method for the determination of zearalenone and its metabolites in grains by capillary electrophoresis. Methods The samples were extracted with liquid liquid partition and ultrasonic extraction, cleaned up with C 18 cartridges, then determined by micellar electrokinetic capillary chromatography(MEKC). Results The limits of detection for Zearalenone(ZON),α Zearalenol(α ZOL),β Zearalenol(β ZOL),Aflatoxin B 1(AFT B 1) and Ochratoxin A(Och A)were 0.0084, 0.081, 0.14, 0.0016 and 0 031 μg/ml, respectively. Recoveries of all compounds were in the range of 77.9% 103.1%. Relative standard deviations (RSDs) were 0.63% 1.98%. Conclusion This sensitive and accurate method can be used in the determination of zearalenone and its metabolites in grains.

关 键 词：玉米赤霉烯酮 真菌毒素 毛细管电泳 

分 类 号：S379[农业科学—农产品加工]