日本血吸虫未知基因JAYL0230全长cDNA的克隆和测序分析  

Choning and Sequencing of a Novel Gene JAYL0230 of Schistosoma japonicum

作　　者：李焱[1] 余新炳[1] 吴忠道[1] 曹爱莲[1] 郑焕钦[1] 李晖婷[1] 包俊英[1] 邵筱[1] 

机构地区：[1]中山医科大学寄生虫学教研室,广州510080

出　　处：《广东寄生虫学会年报》2000年第1期17-20,共4页Journal of Tropical Medicine

基　　金：本课题获得广东省"211工程"重点学科建设课题(98169);国家自然科学基金(30070683)的资助.

摘　　要：为获取日本血吸虫未知基因全长cDNA,通过EST序列的同源性比较,发现日本血吸虫未知基因序列,将未知基因克隆的cDNA插入片段克隆到载体pBluscript（KS）,并对其进行序列测定和序列分析。结果获得日本血吸虫未知基因JAYL0230的克隆,序列分析结果提示该cDNA序列含有一个402bp的完整阅读框序列。To obtain and analyse the complete sequence of a novel gene of Schistosoma japonicum . Methods: A novel gene of Schistosoma japonicum was found by homogenous analysis and was cloned into Blucript(KS), sequence it and analyse it with bioinformatic software. Results: A recombinant plasmid JAYL0230-Bliuscript(KS) was obtained. It contains a complete open reading frame(ORF), and maybe has putative function. Conclusion: Expressed sequence tags strategy and homogenous analysis are efficient to find novel genes.

关 键 词：日本血吸虫 未知基因 克隆 全长CDNA 测序分析 

分 类 号：R383.24[医药卫生—医学寄生虫学]