DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATION FACTOR ON MOUSE ERYTHROLEUKEMIA CELLS  

作　　者：韩代书 赵青 葛晔华 周建平 马静 陈克铨 薛社普 

出　　处：《Chinese Medical Sciences Journal》2002年第4期199-203,共5页中国医学科学杂志（英文版）

基　　金：This work supported by the National Natural Sciences Foundation of China(39670364);This work was originally published in Acta Academiae Medicinae Sinicae(2001;23: 32-35)in Chinese.

摘　　要：Objective. To investigate the roles of mouse erythroid differentiation and denucleation factor (MKI)DF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Method. Mouse erythroleukemia (MEL) cells were transfected with eukaryotie expression plasinid pcl)-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and p-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitolic index,and colony-forming rate in semi-solid medium ( P<0. 01). The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells trarisfected with pcDNA-MEDDF was 3. 43 times higherthan that of control (MEL transfected with blank vector, pcDNA3. 1), and the expression of c-rnyc decreased by66. 3% .Conclusions. MEDDF can induce differentiation of MEL cell and suppress its malignancy.

关 键 词：mouse erythroid differentiation and denucleation factor erythroid differentiation ERYTHROLEUKEMIA 

分 类 号：R733.7[医药卫生—肿瘤]