南、北方坛紫菜多糖结构特征的比较研究  

COMPARATIVE STUDIES ON STRUCTURAL FEATURES OF AGAR POLYSACCHARIDES FROM PORPHYRA HA1T AN EN SIS GROWN IN SOUTH AND NORTH CHINA

作　　者：高洪峰[1] 纪明侯[1] 曹文达[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》1992年第5期541-549,共9页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助 3870137号

摘　　要：对南方产(1987年3月采)和移植北方养殖(1988年11月采)的两种坛紫菜的热水和冷水提取多糖进行了层析分级,用^(13)C-NMR和IR光谱研究了各级分的化学结构特征,结果表明,南北方坛紫菜多糖分别主要由用 1.0mol/L和0.5mol/L NaCl从DEAE—Sephadex A_(50)层析柱洗脱下的带电荷的琼胶糖分子组成;^(13)C-NMR谱图表明坛紫菜主要由琼胶糖结构及其生物前体(硫酸基结合在L-半乳糖的第6位碳上)构成,且甲基化琼胶糖在南方坛紫菜中的含量明显比北移坛紫菜高,这是坛紫菜由南方移植北方后的明显差异。The structural features of agar polysaccharides from Porphyra haitanensis grown in South China and those transplanted to the north were investigated by fractionation on DEAE-Sephadex A50, chemical analysis, infrared and 13C-NMR spectroscopy. The agars, composed mainly of charged molecules, were eluted from DEAE-Sephadex A50 with 1.0 mol/L NaCl solution from the southern P. haitanensis and with 0.5 mol/L NaC1 from the northern one. The 13C-NMR spectra showed that agarobiose [(1→3)-β-D-galactophyranosyl-(1→4)-3, 6-anhydro-α-L-galactophyranose] and the biological precursor of agarobiose [(1→3)-β-D-galactophyranosyl-(l→ 4)-6-sulfate-α-L-galactophyranose] were the major disaccharide repeat units in the charged fractions. The content of the biological precursor in the agar polysaccharides from southern P. haitanensis was higher than that in the northern one; the content of lhe biological precursor extractd by cold water was higher than that extracted by hot water. The content of 6-OMe-D-galactose in the southern P. haitanensis polysaccharides was higher than in the northern one. This distinct difference will be of significance for further study on the physiological characters of P. haitanensis.

关 键 词：坛紫菜 琼胶糖 生物前体 结构 紫菜 

分 类 号：Q949.292.1[生物学—植物学]