反义寡核苷酸对鼻咽癌细胞端粒酶活性的抑制  

Inhibition of the antisense oligodeoxynucleotide targeting against telomerase to telomerase activity in nasopharyngeal carcinoma cells

作　　者：姚红兵[1] 曹前[2] 吴家廉[2] 

机构地区：[1]重庆医科大学附属儿童医院耳鼻喉科,400014 [2]重庆医科大学附属第二医院耳鼻喉科,400010

出　　处：《重庆医学》2003年第4期481-482,共2页Chongqing medicine

摘　　要：目的　检测针对端粒酶的反义寡核苷酸 (AS ODN)对鼻咽癌HNE 1细胞株端粒酶活性的影响。方法　用PCR ELISA法检测细胞内端粒酶活性。结果　鼻咽癌细胞株HNE 1与 5 μmol/L ,2 0 μmol/L反义寡核苷酸共同孵育 1d ,对其端粒酶活性抑制率分别为 3 2 9% ,61 6%。而同浓度正义寡核苷酸 (S ODN)对细胞端粒酶活性无抑制作用。结论　该反义寡核苷酸可抑制鼻咽癌细胞端粒酶活性 。Objective To investigate the influence of AS ODN targeting against telomerase on telomerase activity in nasopharyngeal cacinoma cells.Methods Telomerase activity of HNE 1 cell strain was analysised by PCR ELISA technique.Results HNE 1 cells were incubated with 5μmol/L,20μmol/L AS ODN for 1 day, the inhibition rate of telomerase activity was 32.9%,61.6% respectively.The S ODN did not inhibite telomerase activity of HNE 1 cell strain at the same concentration.Conclusion The AS ODN inhibits telomerase activityof HNE 1 cell strain .This procedure is sequential specific and dose dependence.

关 键 词：抑制 反义寡核苷酸 端粒酶 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]