恶性卵巢上皮肿瘤的P53基因点突变及其意义  

Mutation of P53 Gene in Ovarian Malignant Epithelial Tumors and Its Clinical Significance

作　　者：王振国[1] 朱红[2] 赫朝霞 

机构地区：[1]解放军第309医院妇产科,北京100091 [2]空军北京医院妇产科 [3]全国妇女联合会

出　　处：《中国医师杂志》2003年第4期482-483,共2页Journal of Chinese Physician

摘　　要：目的　为揭示P5 3抗癌基因与卵巢上皮肿瘤发病学的关系 ,对P5 3基因 5 -8外显子突变进行了检测。方法　采用聚合酶链反应 -DNA单链构象多态性分析法 ,以同期良性卵巢上皮肿瘤作对照组。结果　 30例卵巢良性上皮肿瘤中P5 3基因突变 3例 (10 % ) ;而 5 0例恶性卵巢上皮肿瘤中发生突变 19例 (38% ) ,经 χ2 检验 ,差异有显著性 (P <0 0 5 )。但不同组织学分级时P5 3基因点突变差异不显著 (P >0 0 5 )。结论　恶性卵巢上皮肿瘤P5 3基因 5 -8外显子点突变发生率 ,明显高于卵巢良性上皮肿瘤 ,该种突变可能与恶性卵巢上皮肿瘤的发生有关。但P5 3基因 5Objective To evaluate whether P53 gene mutation was related with the pathogenesis of ovarian malignant epithelial tumors or not.Methods P53 gene mutation in exons 5-8 was detected by polymerase chain reaction product-single strand conformation polymorphism analysis (PCR-SSCP).Results The mutation in benign tumors: 3(10%) cases, but in malignant epithelial tumor:19(38%) cases(P<0 05).The statistics does not show significant in different histological grade(P>0 05).Conclusions P53 gene mutation in exons 5-8 was probably related with the pathogenesis of ovarian malignant epithelial tumors, but was not related with histological grade.

关 键 词：卵巢上皮肿瘤 恶性 P53基因 点突变 

分 类 号：R737.31[医药卫生—肿瘤]