尿脱落细胞6号染色体长臂的微卫星改变诊断膀胱肿瘤  

Investigation on microsatellite alteration at chromosome 6q of urine sediment in bladder tumor

作　　者：成凡[1] 楚雍烈[2] 贺大林[3] 杨林[3] 陈萍[2] 杨娥[2] 

机构地区：[1]陕西医学高等专科学校生物化学教研室 [2]医学院微生物学教研室 [3]第一附属医院泌尿外科,陕西西安710061

出　　处：《第四军医大学学报》2003年第8期693-695,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :探讨尿脱落细胞 6q的微卫星改变 (MA)在膀胱肿瘤早期诊断中的应用价值 ,并研究 6q的杂合性缺失(LOH)与膀胱肿瘤的关系 .方法 :应用 6q2 1区域附近D6S4 0 4 ,D6S4 3 4微卫星标志 ,以PCR SSLP 银染法对 3 1例膀胱肿瘤的尿脱落细胞与肿瘤组织进行微卫星分析 .结果 :64 5 %的肿瘤组织和 5 8 1%的尿脱落细胞发生MA ;10例非膀胱肿瘤对照未出现MA ;检出率与肿瘤分期 ,分级无相关性 ;肿瘤组织D6S4 0 4LOH发生率 3 5 5 % ,D6S4 3 4LOH发生率 2 2 6% .结论 :尿脱落细胞微卫星分析有早期诊断意义 ;6q2AIM: To investigate the microsatellite alteration (MA) at chromosome 6q in the early diagnosis of bladder tumor and to study whether the loss of heterozygosity (LOH) at 6q is related to the development of bladder tumor. METHODS: D6S404 and D6434 microsatellite markers near 6q21 were tested by PCR SSLP stain method in both the urine sediment DNA and tumor DNA from 31 cases of bladder tumor. RESULTS: Among the 31 cases of bladder tumor, 64.5% of the tumor tissue DNA and 58 1% of the urine sediments DNA showed MA, while the 10 non bladder tumor cases had no changes at either of the two loci. The result was not associated with the grade or stage of the tumor. LOH was detected in tumor tissues on site for D6S404 (35 5%) and D6S434 (22 6%). CONCLUSION: Microsatellite analysis of urine sediments is of significance in the early diagnosis of bladder tumor and there may be some tumor suppressor gene relevant to the development of bladder tumor near 6q21.

关 键 词：膀胱肿瘤 尿脱落细胞 微卫星改变 6号染体长臂 

分 类 号：R737.14[医药卫生—肿瘤]