Thin Layer Chromatography and Methodological Investigation of Matrine in Zhukuqin Granule  

作　　者：Wei WEI Wenjie ZHONG Juan LIU Rongbin LING 

机构地区：[1]Traditional Chinese Medicine Innovation Laboratory,Veterinary Science Engineering Research Center,Southwest University

出　　处：《Medicinal Plant》2017年第2期4-7,共4页药用植物：英文版

基　　金：Supported by Special Project of National Public Benefit Industry(Agriculture)(201303040-05);Special Project of Science and Technology Groundwork(2013FY110600-03)

摘　　要：[Objectives] To establish a sensitive and simple thin layer chromatography method of matrine in Zhukuqin granule. [Methods]With Zhukuqin granule as control medicinal materials,matrine as reference substance,we chose orthogonal test method,systematically investigated various factors affecting the thin layer chromatography,and selected the best thin layer chromatography conditions. [Results] We screened out the best thin layer chromatography of matrine in the Zhukuqin granule with chloroform as extraction solvent,solvent extraction and ultrasonic extraction as extraction method,toluene-acetone-ethyl acetate-ammonia solution( 2:3:4:0. 5) as developing agent,improvement of bismuth potassium iodide solution as color developing agent. [Conclusions] The separating degree of this method was high,it had good repeatability,and it could be used to control quality of Zhukuqin granule.[Objectives] To establish a sensitive and simple thin layer chromatography method of matrine in Zhukuqin granule. [Methods]With Zhukuqin granule as control medicinal materials,matrine as reference substance,we chose orthogonal test method,systematically investigated various factors affecting the thin layer chromatography,and selected the best thin layer chromatography conditions. [Results] We screened out the best thin layer chromatography of matrine in the Zhukuqin granule with chloroform as extraction solvent,solvent extraction and ultrasonic extraction as extraction method,toluene-acetone-ethyl acetate-ammonia solution( 2:3:4:0. 5) as developing agent,improvement of bismuth potassium iodide solution as color developing agent. [Conclusions] The separating degree of this method was high,it had good repeatability,and it could be used to control quality of Zhukuqin granule.

关 键 词：Zhukuqin GRANULE MATRINE THIN LAYER CHROMATOGRAPHY 

分 类 号：R284[医药卫生—中药学]