Staurosporine对CMK细胞增殖和分化的影响  

The expansion and differentiation effects of Staurosporine on CMK cell

作　　者：赵美蓉[1] 许一多[1] 

机构地区：[1]吉林大学第三医院肿瘤内科,长春130021

出　　处：《中国免疫学杂志》2003年第5期313-314,共2页Chinese Journal of Immunology

摘　　要：目的 :探讨Staurosporine(ST)对原始巨核细胞白血病细胞系 (CMK)的增殖分化作用。方法 :采用MTT分析方法及流式细胞仪测定细胞周期分布。结果 :ST呈浓度依赖性地抑制CMK细胞的增殖 ,IC50 为 5 0nmol L ;ST可将CMK细胞阻抑在G1 期 ,从而减少S和G2 M期的细胞 ;以巨核细胞分化特异性膜表面抗原CD41为指标 ,80nmol L的ST可使CMK细胞CD41抗原表达率明显增加。结论 :ST可抑制CMK细胞的增殖 ,诱导其分化 ,ST作为一种新的抗肿瘤因子具有与其他抗肿瘤药物不同的作用机制。Objective:To determine the expansion and differentiation effect of Staurosporine on human megalaryoblastic cell line(CMK).Methods:With MTT and colony assaies.Results:Staurosporine could densitily inhibited the expansion of CMK cell,IC 50 was 50 nmol/L,Staurosporine could inhibite CMK cell at G 1 phase,then decreased the cells of S and G 2M phases,ST in 80 nmol/L improve the expression of CD41 antigen in CMK cell,induce its differentiation.Conclusion:ST as a new kind of anti-tumor facor has different mechanism with other anti-tumor drugs. [

关 键 词：STAUROSPORINE CMK细胞增殖 分化 影响 

分 类 号：R730.3[医药卫生—肿瘤]