从L-阿拉伯胶提取L-阿拉伯糖  

Extraction and Purification of L-Arabinose from Arabic Gum

作　　者：冯亚青[1] 刘燕[1] 张晓东[1] 张安训[1] 张卫红[1] 

机构地区：[1]天津大学化工学院精细化工系C1化学与化工国家重点实验室,天津300072

出　　处：《精细化工》2003年第5期288-290,共3页Fine Chemicals

摘　　要：阿拉伯胶在硫酸作用下水解得到含L 阿拉伯糖的混合液,经w(C2H5OH)=90%的乙醇萃取,再经工业乙酸沉淀,可得粗L 阿拉伯糖。正交实验确定了酸性水解反应的影响因素和最佳条件为:水解温度90～100℃,w(H2SO4)=1 0%,L 阿拉伯糖的粗收率为85%,质量分数为66 7%。经双柱色谱分离提纯L 阿拉伯糖,所用洗脱剂分别为:V(正丁醇)∶V(乙酸乙酯)∶V(异丙醇)∶V(乙酸)∶V(水)=3 5∶10∶6 5∶3∶3和V(乙酸乙酯)∶V(异丙醇)∶V(水)=5∶2 2∶1 1,得到收率大于50%,质量分数为99%的L 阿拉伯糖。Arabic gum was hydrolyzed with sulfuric acid solution to give the mixed solution containing L-arabinose,which was concentrated and extracted with 90% alcohol and precipitated by industrial acetic acid to give the crude L-arabinose.The influencing factors were studied by orthogonal test to get the optimum conditions for acidic hydrolysis,i.e.,hydrolyzing temperature 90-100 ℃ and w(H2SO4)=1.0%.Yield of crude L-arabinose was 85% and the purity was 66.7%.After chromatographic separation with double column,purified L-arabinose was obtained.Two eluting reagent systems were chosen,i.e.,V(n-butanol)∶V(ethyl acetate)∶V(iso-butanol)∶V(acetic acid)∶V(water)=3.5∶10∶6.5∶3∶3 and V(ethyl acetate)∶V(iso-butanol)∶V(water)= 5∶2.2∶1.1.The yield was more than 50% and the purity was 99%.

关 键 词：L-阿拉伯糖 阿拉伯胶 提取 双柱色谱分离 

分 类 号：Q53[生物学—生物化学]