软组织平滑肌肉瘤染色体3p位点特异性的杂合性缺失  

Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma

作　　者：彭惠[1] 杨光华[1] 步宏[1] 郭立新[1] 

机构地区：[1]四川大学华西医院病理科

出　　处：《中华病理学杂志》2003年第2期124-127,共4页Chinese Journal of Pathology

基　　金：纽约中华医学基金会 (CMB)基金资助项目 (0 0 72 2 )

摘　　要：目的　对软组织平滑肌肉瘤 (LMS)染色体 3p上的 5个特异性位点进行微卫星多态性分析 ,以明确这些位点染色体等位基因杂合性缺失 (LOH)情况。方法　采用微卫星DNA 聚合酶链反应 银染法分析 2 2例LMS 3p14 .2 pter范围内的 5个特异性位点LOH状态。结果　 4 5 4 %LMS至少在一个位点出现LOH ,以D3s12 95最高 (36 8% ) ,其次为D3s12 89(10 5 % ) ,而D3s12 93未检测到LOH。不同组织学分级、大小及不同部位的LMS其LOH发生差异无显著性。结论　LMS 3p14 2 2 3范围发生杂合性缺失率较高 ,D3s12 95及D3s12Objective To analyze the loss of heterozygosity (LOH) at 5 loci on chromosome 3p in soft tissue leiomyosarcoma (LMS) Methods LOH was detected in 22 cases of LMS using PCR silver staining targeting 5 microsatellite sites on 3p14 2 pter Relation between LOH and LMS clinical pathological features was also analyzed. Results Ten of 22 LMS samples showed LOH at more than one locus (45 4%) Among the 5 loci, LOH occurred more frequently at D3s1295 (36 8%) and D3s1289 (10 5%), but absent at D3s1293 No significant difference was found on LOH incidence between different grade, size and location of LMS Conclusions LOH on chromosome 3p14 2 23 region is relatively frequent in LMS Region around D3s1295 and D3s1289 may harbor tumor suppressor gene relating to LMS

关 键 词：软组织平滑肌肉瘤 染色体3p位点 特异性 杂合性缺失 

分 类 号：R738.6[医药卫生—肿瘤]