钼酸铵分光光度法测定葡萄籽提取物中的原花青素  

Determination of Functional Components in Grape Seeds by Ammonium Molybdate Spectrophotometry

作　　者：马亚军[1] 杨秉勤[1] 郎惠云[1] 

机构地区：[1]西北大学化学系,西安710069

出　　处：《食品科学》2003年第5期135-137,共3页Food Science

摘　　要：本文提出了测定原花青素的新方法,它是基于原花青素与钼酸铵在弱酸性介质中反应生成黄色钼酸酯,反应产物在333nm波长处具有最大吸收。该方法的线性范围为5～110μg/ml,回收率为97.8%～102.5%,相关系数r=0.9995,检出限为2.7μg/ml.A new method for deter mining oligomeric procyanidins by Ammonium Molybdate Spectrophotometry was studied.This basic reaction of oligomeric procyanidins with Ammonium molybdate to produce yellow ester molybdate would bepresented in a weak acid solution .The linear region was 5～110μg/ml, RSD=1.1%～1.3% and the recovery was 97.8%～102.5%. Thus the method was applied to determine oligomeric procyanidins to obtain a good result.

关 键 词：钼酸铵分光光度法 测定 葡萄籽提取物 原花青素 黄色钼酸酯 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学]