多肽的反相梯度加压毛细管电色谱分离  

Separation of Polypeptides by Gradient Pressurized Capillary Electrochromatography

作　　者：张锴[1] 阎超 江正瑾[1] 张智超[1] 王琴孙[1] 高如瑜[1] 

机构地区：[1]南开大学化学学院元素有机化学重点实验室,天津300071 [2]通微分析仪器技术有限公司,上海201203

出　　处：《分析测试学报》2003年第3期32-34,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目 (20175010) ;元素有机化学国家重点实验室第十四期开放基金资助项目 (201203)

摘　　要：以C18 为固定相 ,采用电压和压力联合驱动流动相 ,研究反相加压毛细管电色谱分离多肽 ;考察了加压电色谱中 ,电压对带电和中性物质迁移的影响 ,实现了梯度加压毛细管电色谱分离6种多肽 ;结果表明 ,加压电色谱可以很好地抑制气泡形成 ,实验结果准确 ,重复性好 ;梯度加压毛细管电色谱在复杂样品的分离分析中 。Separation of polypeptides was investigated by reversed _ phase pressurized capillary electrochromatography (pCEC) with ODS stationary phase. The effect of voltage on migration behaviors of charged polypeptides and methanol in pCEC was studied. Separation of 6 polypeptides was performed by gradient pCEC on a new CEC instrument Trisep 2000 GV. The separation results showed that the bubble formation in column was suppressed and repeatability of experiment data was good for pCEC. Pressurized capillary electrochromatography with gradient elution may have great potential for separating a wide variety of complex samples.

关 键 词：多肽 梯度洗脱 毛细管电色谱 分离 加压电色谱 

分 类 号：Q516[生物学—生物化学] O629.72[理学—有机化学]