云南省马铃薯晚疫病菌的交配型、抗药性及生理小种分布的研究(英文)  

Mating Type, Fungicide Sensitivity and Physiological Race of Phytophthora infestans Collected from Yunnan Province

作　　者：K.Y.RYU 罗文富[2] 杨艳丽[2] 国立耘[3] 郭华春[4] 王毅[5] 陈海如[2] 

机构地区：[1]韩国江原道高岭地国家农业实验站 [2]云南农业大学植物病理系,昆明650201 [3]中国农业大学植物病理系,北京100094 [4]云南农业大学农业与生物技术学院,昆明650201 [5]国际马铃薯中心北京办事处,北京100081

出　　处：《植物病理学报》2003年第2期126-131,共6页Acta Phytopathologica Sinica

基　　金：Yunnan Natural Science Foundation(97CO23M);President Foundation of Yunnan Agricultural University;National Natural Science Foundation(39970480)

摘　　要：从云南省陆良县、建水县和南涧县采集2000年秋播的马铃薯晚疫病标本,分离获得124个致病疫霉菌株,连同1999～2000年分离自昆明、曲靖等地的春播马铃薯晚疫病的10个菌株共134个,采用常规技术对这些菌株进行了交配型、抗药性及生理小种的研究。交配型测定结果为:陆良县的18个菌株中有3个为A_2交配型,其余15个菌株为A_1交配型,建水县和南涧县的106个菌株及昆明、曲靖1999～2000年的10个菌株都是A_1交配型。测定了83个菌株对瑞毒霉(metalaxyl)和烯酰吗啉(dimethomorph)的抗药性,结果有12.0％的菌株对瑞毒霉表现抗性,16.9％表现中抗,71.1％表现敏感,其中陆良、昆明和曲靖的抗瑞毒霉菌株的比例大于建水和南涧的抗药性菌株的比例;所测定的83个菌株都对烯酰吗啉表现敏感。生理小种鉴定结果表明,云南晚疫病菌为3.4、0、3和4生理小种,分别占鉴定菌株总数的48.0％、32.5％、15.6％和3.9％。但南涧仅发现3.4和0小种,而昆明有0、3、3.4和4小种,陆良有0、3和3.4小种,可见昆明和陆良地区的晚疫病菌生理小种比南涧地区的生理小种的组成更趋多样。The mating type, chemical response and physiological race of Phytophthora infestans collected from Yunnan Province were determined. In total, 134 isolates of P. infestans were used in this study, among which 124 isolates collected from potato planted in autumn of 2000 in Luliang, Jianshui, Nanjian areas and 10 isolates collected from potato planted in spring of 1999 - 2000 in Kunming and Qujing areas. Among 18 isolates collected from Luliang County, 3 isolates belonged to A_2 mating type, while 15 isolates belonged to A_1 mating type. Only A_1 mating type was detected in 106 isolates collected from potato in autumn of 2000 in Jianshui, Nanjian and in 10 isolates collected from 1999 to 2000 in Kunming and Qujing areas. The response of 83 isolates to metalaxyl showed that 71.1 % were sensitive, 16.9% were intermediate resistant, and 12 .0% were resistant. The percentage of metalaxy sensitive isolates is higher in Luliang, Kunming and Qujing than in Nan- jian and Jianshui. All of 83 isolates detected were sensitive to dimethomorph. Results also showed that the physiological races in Yunnan Province consisted of races 3 .4, 0, 3 and 4, representing 48.0%, 32.5%, 15.6% and 3.9% of the population, respectively. However, only 0 and 3 .4 races were detected in Nanjian, while races 0, 3, 3 .4 and 4 races were detected in Kunming and races 0, 3 and 3 .4 were detected in Luliang. This indicated tha population of P. infestans in Kunming and Luliang areas is more diversified in race composition than tha in Nanjian area.

关 键 词：云南 马铃薯 晚疫病菌 交配型 抗药性 生理小种 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]