贲门癌与食管癌组织p53蛋白表达和基因突变分析  

p53 protein accumulation and p53 gene mutation in esophageal and gastric cardia cancer from the patients at Linzhou, Henan

作　　者：周琦[1] 郑作昱[1] 王立东[1] 刘宾[2] 秦艳茹[1] 王道存[1] 常志伟[1] 易会兴[1] 范宗民[1] 李吉林 

机构地区：[1]郑州大学医学院癌症研究室,郑州450052 [2]首都医科大学同仁医院消化内科,北京100013 [3]林州市姚村食管癌医院病理科,林州456592

出　　处：《郑州大学学报（医学版）》2003年第3期313-317,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家杰出青年科学基金3 0 0 2 5 0 16;河南省高校创新人才工程基金;河南省医药卫生创新人才工程基金;国家自然科学基金资助项目

摘　　要：目的 :探讨河南食管癌高发区人群贲门癌和食管癌组织p5 3基因突变和蛋白聚集的变化规律 ,进一步深入了解食管癌癌变机制。方法 :采用PCR SSCP、DNA测序分析和免疫组织化学染色方法对 19例贲门腺癌手术切除标本和 5 0例食管鳞癌手术切除标本的p5 3基因突变和蛋白聚集的变化进行检测。结果 :①食管鳞癌和贲门腺癌组织中p5 3蛋白过度表达的阳性率分别为 70 %和 6 8% ,p5 3基因突变率分别为 5 8%和 5 3% ,p5 3基因突变和蛋白过度表达的一致率分别为 6 4 %和 4 2 % ;②食管鳞癌组织p5 3基因突变在第 5～ 8外显子中均有分布 ,而在贲门腺癌组织集中分布于第 5和第 7外显子 ;③对p5 3基因突变谱分析显示 ,在食管鳞癌和贲门腺癌组织中 ,G :C→A :T是最常见的碱基突变类型 (4 8%和 30 % ) ,碱基的插入和缺失在贲门腺癌中也较为常见。结论 :p5 3基因在食管鳞癌和贲门腺癌组织中的相似变化提示林州地区贲门和食管癌可能存在共同的致病危险因素 ;对p5 3蛋白检测能从一定程度上反映p5Aim: To characterize p53 protein accumulation and p53 gene mutation in esophageal and gastric cardia cancer from the patients at Linzhou, Henan, to further understand the molecular mechanism of these cancer.Methods:PCR SSCP, DNA sequencing and immunohistochemistry (IHC) were used to determine the p53 gene mutation and protein accumulation in 50 samples of surgically resected esophageal squamous cell carcinoma and 19 samples of surgically resected adenocarcinoma of the gastric cardia.Results:①In esophageal squamous cell carcinoma(SCC) and gastric cardia adenocarcinoma(GCA), the frequencies of the p53 positive immunostaining were 70% and 68%, respectively; ②The frequencies of p53 gene mutation were 58% and 53% in SCC and GCA, respectively; ③In SCC, the p53 gene mutations distributed from exon 5 to exon 8, but the p53 gene mutations clustered in exon 5 and exon 7 in GCA; ④G:C→A:T transition was the most frequent p53 gene mutation type both in SCC and GCA (48% and 30%, respectively). The deletion/insertion of p53 gene was also common in GCA; ⑤The consistency of p53 gene mutation and protein accumulation were 64% and 42% respectively in SCC and GCA.Conclusion:The similar changes in p53 gene mutation and protein accumulation suggest that SCC and GCA in Linzhou may share similar environmental risk factors and that the status of p53 protein expression to some extent may represent the status of p53 mutation.

关 键 词：贲门癌 食管癌 P53蛋白 表达 基因突变 分析 

分 类 号：R735.1[医药卫生—肿瘤]