甜味蛋白thaumatin表达载体的构建  

Construction of the Expressing Vector of Thaumatin

作　　者：赵琦[1] 孔建强[1] 高音[1] 

机构地区：[1]首都师范大学生物系,北京100037

出　　处：《首都师范大学学报（自然科学版）》2003年第2期64-67,共4页Journal of Capital Normal University：Natural Science Edition

基　　金：国家自然科学基金资助 (A3 0 0 70 488)

摘　　要：将克隆在不同质粒上的thaumatin基因的两个片段连接起来 ,连接产物克隆到质粒pUC18上 .测序结果表明 ,连接产物是一个完整的thaumatincDNA基因 .将此基因通过基因重组技术 ,克隆到植物表达载体pBI12 1中 ,构建了一个新的转化植物的表达载体—pBI12 1 tha.After cheated with T\-4 ligase, the two fragments which were cloned into two different plasmids were ligated into a complete thaumatin gene. The traget gene was confirmed by sequencing. By DNA recombinatnt methods, the cDNA gene was cloned into vector pBI121.A new expressing vector\_\_pBI121\|tha was successfully constructed.

关 键 词：甜味蛋白 thaumatin基因 基因重组 基因克隆 植物表达载体 基因工程 

分 类 号：Q782[生物学—分子生物学] Q51