微生物发酵法生产乳清酸  

Studies on the Orotic Acid Production by Fermentation

作　　者：张一平[1] 柏建新[1] 朱晓宏[1] 杜郭君[1] 邓崇亮[1] 

机构地区：[1]江苏省微生物研究所,江苏无锡214063

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第3期32-35,40,共5页Journal of Wuxi University of Light Industry

基　　金：江苏省社会发展基金项目(编号BS2000094)资助课题.

摘　　要：以谷氨酸棒杆菌JSIM 201为出发菌株,通过紫外线和甲基磺酸乙酯诱变处理,得到了一株尿嘧啶营养缺陷型突变体U 12菌株,它能以葡萄糖为碳源,硫酸铵为氮源,在发酵液中积累一种紫外吸收物质.该紫外吸收物质经物理、化学分析鉴定,证明是乳清酸.对U 12菌株进行了发酵条件优化研究,在最佳发酵工艺条件下,积累乳清酸最高达到8.6g/L.The corynebacterium glutamicum strain JSIM201 was selected as the starting strain. By treatment of UV ray and EMS, an uracil auxotrophy mutant strain U12 was obtained. Using glucose and ammonium sulfate as the carban source and nitrogen source, the mutant could accumulate an ultraviolet absorption substance in its cultural broth. This substance isolated from its cultural broth was identified as orotic acid by physical and chemical analysis. The fermentation studies on strain U12 was conducted under the optimal fermentation conditions, and the largest orotic acid yield was 8.6 g/L.

关 键 词：微生物发酵法生产 乳清酸 谷氨酸棒杆菌 尿嘧啶营养缺陷型突变体U-12菌株 诱变 工艺优化 

分 类 号：TQ924[轻工技术与工程—发酵工程]