饲用黑曲霉纤维素酶的纯化及酶学特性的研究  

Purification and Properties of the Cellulase from Aspergillus Niger

作　　者：王金华[1] 陈雄[1] 李世杰[1] 邱雁临[1] 

机构地区：[1]湖北工学院,湖北武汉430068

出　　处：《粮食与饲料工业》2003年第6期28-30,共3页Cereal & Feed Industry

摘　　要：从饲用黑曲霉纤维素酶的粗酶粉中 ,可以分离纯化 4种内切 β 1,4 葡聚糖酶 (EG1、EG2 、EG3和EG4)。经SDS -PAGE凝胶电泳分析 ,分子量分别为 6 75 0 0、5 930 0、4 770 0和 3810 0u。EG4酶的最适作用温度为 5 5℃ ,其他 3种酶均为 5 0℃。而其酶活相对稳定温度范围为 35～ 6 5℃。EG3 和EG4的耐热性优于EG1和EG2 。它们的最适pH值均为 5 .0 ,在pH值为 2 .5～ 7.5情况下都较稳定。在pH值大于 8.0时 ,相对酶活力下降较快 ,其中EG1和EG4失活程度高于EG2 和EG3 。Four kinds of cellulase (endo-β-glucanases EG 1, EG 2, EG 3 and EG 4) were purified from Aspergillus niger. The molecular weights of them were 67 500, 59 300,47 700 and 38 100 as estimated by SDS gel electrophoresis. All of the EG 1, EG 2, EG 3 and EG 4 showed optimal activity at pH 5.0 and at the temperature 50 ℃, 50 ℃, 50 ℃ and 55 ℃ respectively. They were relatively stable in the pH range of 2.5～7.5 and the temperature of 35～65℃. When pH value exceeds 8.0, the relative activity of the cellulase decreases relatively quickly, and the inactivation degree of EG 1 and EG 4 was higher than that of EG 2, and EG 3.

关 键 词：饲用 黑曲霉纤维素酶 纯化 酶学特性 提取 粗酶粉 

分 类 号：S816[农业科学—饲料科学]