PCR Amplication and Sequencing of ITS-2 rDNA of Leucocytozoon caulleryi  

作　　者：LIGuo-qing XUEHong ZHUXing-quan 

机构地区：[1]CollegeofVeterinaryMedicine,SouthChinaAgriculturalUniversity,Guangzhou510642,P.R.China [2]DepartmentofVeterinaryScience,TheUniversityofMelbourne,Victoria3030,Autralia

出　　处：《Agricultural Sciences in China》2003年第5期573-577,共5页中国农业科学（英文版）

基　　金：supported by grants from National Natural Science Foundation of China(39870549);Natural Science Foundation of Guangdong Province(980134).

摘　　要：The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).

关 键 词：Leucocytozoon caulleryi ITS-2 PCR CLONING SEQUENCING 

分 类 号：S858.31[农业科学—临床兽医学] S852.7[农业科学—兽医学]