小鼠GM-CSF的基因克隆和锚定修饰  

Cloning mouse GM-CSF and construction of GPI-anchored mGM-CSF

作　　者：于继云[1] 陈兴[1] 蔡欣[1] 田蓉[2] 程绍辉[1] 许红莲[1] 王嘉玺[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]第四军医大学西京医院皮肤科,西安710032

出　　处：《生物技术通讯》2003年第3期178-179,共2页Letters in Biotechnology

基　　金：国家863项目(2001AA217131)

摘　　要：将糖基化磷脂酰肌醇(GPI)锚定修饰的细胞因子直接转移到肿瘤细胞膜上,是研究治疗性肿瘤疫苗的一种有潜力的新策略。克隆了小鼠GM-CSF基因,并通过将从衰变加速因子(DAF)来源的GPI修饰性信号序列与mGM-CSF嵌合,获得了GPI修饰的mGM-CSF分子,构建并鉴定了GPI锚定修饰的真核表达载体pCI/GPI-mGM-CSF,为进一步研究表面工程治疗性肿瘤疫苗奠定了基础。Direct modification of tumor cell membranes by transfer of a glycosyl-phosphatidylinositol(GPI)-anchored cytokine has been proposed as a novel potentially useful strategy to development of therapeutic tumor vaccines.In this study,mouse GM-CSF was amplified by RT-PCR using the total RNA extracted from BALB/c mouse spleen cells activated with IL-2and anti-CD3antibody in vitro.Glycosylphosphatidylinositol(GPI)-anchored variants of mGM-CSF were produced via chimerization with alternative GPI-modification signal sequences from decay-accelerat-ing factor(DAF).GPI-anchored mGM-CSF eukaryotic expression vector pCI /GPI-mGM-CSF was contracted and iso-latedand,it will may be used for research of new type therapeutic tumor vaccines.

关 键 词：小鼠 GM-CSF基因 克隆 锚定修饰 肿瘤疫苗 糖基化磷脂酰肌醇 蛋白质修饰 细胞膜 

分 类 号：Q26[生物学—细胞生物学] R73[医药卫生—肿瘤]