喉鳞状细胞癌P53蛋白表达和基因突变  

Alteration of P53 protein expression and p53 gene in laryngeal carcinoma

作　　者：朱明[1] 林山[2] 李君[2] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2003年第3期237-240,共4页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的 :探讨喉鳞癌 P5 3蛋白表达和基因移码突变情况。方法 :采用 SP法检测 31例喉鳞癌的 P5 3蛋白表达 ;人工微切割 - PCR-异源双链分析法检测 11例 P5 3蛋白阴性标本第 5、6、7、8外显子的移码突变。结果 :免疫组织化学显示 P5 3总阳性率为 6 4.5 %。 T1、T2期阳性率 4 3.8% ,T3、T4期为 86 .7% ;有和无淋巴结转移的分别为91.7%、4 7.4 % (P<0 .0 5 ) ;高分化、中分化及低分化鳞癌中 P5 3蛋白阳性率分别为 4 5 .5 % (5 / 11)、6 6 .7% (8/ 12 )、87.5 % (7/ 8)。异源双链分析法分析 1例第 5外显子和 1例第 7外显子阳性。结论 :在喉鳞癌中 ,P5 3蛋白阳性表达多见于 T3及 T4期肿瘤及有颈淋巴结转移者 ;p5 3基因突变率随肿瘤分化程度的降低而升高。Objective: To study the prevalence of P53 protein expression and p53 gene mutation in laryngeal carcinoma.Methods:Using immunohistochemistry P53 expression was detected in 31 patients with laryngeal carcinoma.In 11 P53 negative patients,microdissection-PCR-HA technique was used to determine mutation in p53 exon 5,6,7,8. Results:Among the 31 patients tested with immunostaining, the overall average positive rate was 64.5%. Positive rates for T3 and T4 tumors were 86.7% vs 43.8% in T1 and T2 tumors.The positive rate was 91.7% in those with cervical node metastasis vs 47.4% in those without lymph node metastasis. The positive P53 immunostaining was more frequently found in poor-differentiated carcinoma (87.5%) and moderate-differentiated carcinoma (66.7%),than in well-differentiated carcinoma(45.5%).The abnormal exon 5 or 7 of p53 gene were detected in 2 out of 11 cases,in which P53 was negative.Conclusion:P53 gene mutation is related with TNM grading and cervical lymph node metastasis in laryngeal carcinoma.P53 mutation tents to be correlated to pathologic grading.

关 键 词：喉鳞状细胞癌 P53蛋白 基因突变 基因表达 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]