反复分批发酵法生产甘油研究  

Glycerol Production of Repeated Batch Fermentation Technology with Free Osmophilic Yeast

作　　者：刘永强[1] 刘德华[2] 刘天中[1] 马志国[2] 

机构地区：[1]中国科学院化工冶金研究所生化工程国家重点实验室,北京100080 [2]清华大学化工系应用化学研究所,北京100084

出　　处：《化学工程》2003年第3期50-52,57,共4页Chemical Engineering(China)

摘　　要：研究了反复分批发酵法在耐高渗酵母生产甘油中的应用 ,通过对比可知 ,游离耐高渗酵母的反复分批发酵性能优于固定化耐高渗酵母的反复分批发酵 ,且在反复分批发酵 1 2个批次后 ,其发酵性能依然稳定。由对游离细胞反复分批发酵的动力学参数分析可知 ,反复分批发酵法之所以具有良好的发酵性能 ,主要是由发酵液中高的菌体密度决定的。Glycerol production of repeated batch fermentation technology was investigated. After fermented repeatedly for twelve cycles, the free cells of osmophilic yeast could be and still showed the excellent stability. In comparison with immobilized cells of osmophilic yeast, free cells could result in better yield, productivity and operation stability during repeated batch fermentation. According to analysis of kinetics, the reason of better performance of repeated batch fermentation with free cells was higher cell density in fermentive liquid.

关 键 词：反复分批发酵法 甘油 耐高渗酵母 固定化 动力学参数 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]