琼胶低聚糖清除自由基的活性  

Radical scavenging ability of agar oligomers

作　　者：薛长湖[1] 徐强[1] 赵雪[1] 李兆杰[1] 林洪[1] 

机构地区：[1]青岛海洋大学食品科学与工程系,山东青岛266003

出　　处：《水产学报》2003年第3期283-288,共6页Journal of Fisheries of China

基　　金：山东省优秀中青年科学家科研奖励基金(991206008)

摘　　要：采用体外鲁米诺发光和DPPH体系对琼胶低聚糖清除氧自由基活性进行了研究。实验结果表明,琼胶低聚糖ML对水溶性的·OH和O2·-有很好的清除作用,IC50分别为0.05mg·mL-1、0.7mg·mL-1,效果与自由基清除剂肌肽相近。琼胶低聚糖ML对醇溶性的DPPH自由基有一定的清除活性(IC50为6.4mg·mL-1),但是清除效果远不如Vc和丙丁酚。对不同的聚合度、含硫量和摩尔浓度的琼胶低聚糖的清除O2·-的活性进行比较,实验发现含硫量高、分子量大的低聚糖在清除自由基过程中发挥主要作用。琼胶低聚糖ML对LPS+D-GalN急性肝损伤小鼠的实验表明,一定浓度的ML灌胃可以有效抑制肝损伤小鼠血清GPT活性和MDA浓度的升高,保护抗氧化酶SOD和GSH Px的活力,对肝损伤有很好的保护作用。The free oxygen radical scavenging effects of agar oligomers were studied by the method for determination of Luminesecence and DPPH assay. The results showed that agar oligomer ML had strong scavenging effects on ·OH and O2·-, IC50 were 0.05mg·mL-1 and 0.7mg·mL-1, but the scavenging effect on DPPH radical was much lower than Vc and probutol. Comparation of the scavenging activities on O2·- between agar Oligomers with different sulphate content and molecular weight showed that high sulphate group or high molecular weight took the main action to scavenge radicals. Agar oligomers taken ig to LPS+D-GalN induced liver injury mice could effectively inhibit the inrease of GPT acticity and MDA content, and maintain the acitivities of SOD?GSH-Px activity in mice serum. It had good protective effect on liver injury.

关 键 词：生物机体 肝脏功能 水产药物学 琼胶低聚糖 自由基活性 清除技术 鲁米诺发光 DPPH体系 含硫量 自由基清除剂 

分 类 号：S948[农业科学—水产养殖]