荧光原位杂交在环境微生物学中的应用及进展  

Application and Progress of Fluorescence In Situ Hybridization (FISH) in Environmental Microbiology

作　　者：邢德峰[1] 任南琪[1] 李建政[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090

出　　处：《环境科学研究》2003年第3期55-58,共4页Research of Environmental Sciences

基　　金：国家杰出青年科学基金资助项目;国家973重点基础研究资助项目(G2000026402)

摘　　要：荧光原位杂交技术广泛用于分析复杂环境的微生物群落构成,可以在自然生境中监测和鉴定微生物,并能对未被培养的微生物进行检测。rRNA为靶序列寡核苷酸或PNA探针的荧光原位杂交能提供微生物的形态学、数量、空间分布等信息,现已成为环境科学研究领域中的热点技术。对荧光原位杂交技术的发展和在环境微生物学中的应用进行了综述,探讨了该技术的应用和发展前景。Fluorescence in situ hybridization(FISH) has been widely applied for analyzing the composition of microbial communities in complex environmental samples.The examination and identification of individual microbial cells within their natural habitat could be performed,and those ye\|to\|be cultured microorganisms could be visualized by this technology.The information about morphology,number and spatial distribution of the microorganisms could be provided by FISH within rRNA\|targeted oligonucleotide probes or PNA.Furthermore,it is a powerful tool for studying environmental microbiology.The authors reviewed the major techniques and progresses of FISH,and its applications in microbial ecology,and subsequently discussed the perspectives of FISH.

关 键 词：荧光原位杂交 16SrBNA 寡核苷酸探针 PNA 环境微生物 微生物生态学 

分 类 号：X172[环境科学与工程—环境科学]