黑曲霉液体发酵产纤维素酶酶系均衡性研究  

Characteristics of cellulase systems induced by aspergillus niger710712

作　　者：吴石金[1] 郑丽琴[1] 夏一峰[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2003年第4期439-443,共5页Journal of Zhejiang University of Technology

基　　金：青年发展基金科研资助项目 (Χ10 5 2 0 13)

摘　　要：采用 p H值和温度两因素用正交实验法分析出黑曲霉 71 0 71 2纤维素酶系各组分最适酶解条件 ,其组合分别为 :C1酶 ( p H5.0 ,45℃ ) ,Cx酶 ( p H4.0 ,60℃ ) ,βG( p H5.0 ,65℃ )。在液体发酵条件下 ,探讨缓冲系统以及 Ca Cl2 及吐温 -80对产酶的影响 ;分析了不同碳源以及碳源与碳源的配比对黑曲霉产纤维素酶系均衡性的相关性。结果表明碳源的种类和配比对最大酶活及其形成时间、比酶活和分泌变化规律均有不同影响效应。纤维素粉和麦麸浓度配比为 1 .0 %和 2 .0 %时可明显改善所产纤维素酶系的均衡性 ;用 0 .1 M柠檬酸 -磷酸氢二钠缓冲液和添加适量 Ca Cl2 ( 0 .3 % )和吐温 -80 ( 0 .3 % )对产酶有正向影响。By adopting orthogonol tests, the optimal enzymatic hydrolytic conditions of the cellulase system of aspergillus niger710712 are found to be pH 5.0,45 ℃ for enzyme C\-1,and pH 4.0,60 ℃ for enzyme Cx and pH 5.0,65 ℃ for enzymeβG. Under the condition of liquid state fermentation, we studied the influence of CaCl\-2 and Tuwen-80 on the production of cellulase systems and the influence of C\|resource on the balance of the cellulase system produced by aspergillus niger710712. Results showed that there exist certain effects on the balance of cellulase system secretion coinciding with different C\|resource and the dose volumes of C\|resource about the cellulase system. The combination of CaCl\-2(0.3%) and Tuwen-80(0.3%) also shows a certain effect on the secretion of enzymes.

关 键 词：黑曲霉纤维素酶系 均衡性 pH值 温度 正交实验 酶解条件 碳源 真菌纤维素酶系 酶活 

分 类 号：Q556[生物学—生物化学]