喉癌染色体6q25区域的杂合性丢失和微卫星不稳定性分析  

Loss of Heterozygosity and Microsatellite Instability in Chromosome 6q25 Region of Laryngeal Carcinoma

作　　者：康宁[1] 徐振明 李福才[1] 高红明 富伟能[1] 孙开来[1] 

机构地区：[1]中国医科大学基础医学院医学遗传学教研室 [2]空军第463医院耳鼻喉科

出　　处：《中国医科大学学报》2003年第4期289-291,共3页Journal of China Medical University

基　　金：国家自然科学基金项目 (3 0 1710 0 8);辽宁省自然科学基金资助项目 (2 0 0 110 10 3 9)

摘　　要：目的 :在 6 q2 5区域内筛查喉癌相关的基因 ,探讨胰岛素样生长因子受体Ⅱ (IGF2R)基因及脆性位点FRA6E在喉癌发生中的作用。方法 :在 6 q2 5区域选择 3个微卫星多态标记 ,其中D6S980位于IGF2R的 5’端且与FRA6E紧密连锁。应用聚合酶链式反应 聚丙烯酰胺尿素凝胶电泳 DNA银染方法对 70例喉癌进行杂合性丢失(LOH)和微卫星不稳定性 (MI)分析。结果 :在这 3个痤位上 ,喉癌LOH频率以D6S980处最高 ,达 4 1.4 % ,MI频率为 2 6 .8%。总的异常频率为 6 8.2 %。LOH和MI与喉癌临床分期无关。结论 :提示IGF2R可能是喉癌相关的抑癌基因。Objective: Our aims were to search for gene closely related to pathogenesis of laryngeal carcinoma in chromosome 6q25 region and to study the correlation of insulin-like growth factor Ⅱ recepto (IGF2R) and FRA6E with laryngeal carcinoma. Methods: Three microsatellite polymorphism markers in 6q25 were selected to detect loss of heterozy gosity (LOH) and microsatellite instability (MI) in 70 cases of laryngeal carcinoma by using PCR-PAUGE (polymerase chin reaction-polyacrylamide urea gel electrophoresis)-DNA silver staining method.. Results: Among D6S1708,D6S437,and D6S980,the highest frequency of LOH was in D6S980 (41.4%),the MI frequency was 26.8%,with the highest frequency (26.8)% in D6S980.The overall abnormal frequency was 68.2% in D6S980.Furthermore,the frequencies of both LOH and MI were not related to the type of laryngeal carcinoma.Conclusion: The IGF2R might be and important tumor suppressor gene (TSG) in laryngeal carcinoma and the chromosomal region of FRA6E might harbor a putative TSG related to laryngeal carcinoma.

关 键 词：喉癌 胰岛素样生长因子受体-Ⅱ基因 脆性位点FRA6E 杂合性丢失 微卫星不稳定性 

分 类 号：R739.65[医药卫生—肿瘤]