9种金属离子对缢蛏消化酶活力的影响  

Effects of nine metal ions on digestive enzyme activities of Sinonovacula constricta

作　　者：吴众望[1] 潘鲁青[1] 董双林[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《中国水产科学》2003年第4期297-300,共4页Journal of Fishery Sciences of China

基　　金：国家重点基础研究规划项目(G1999012011)

摘　　要：以酶学分析的方法研究9种金属离子(Cu2+、Mg2+、Fe3+、Hg2+、Zn2+、Ca2+、Pb2+、Ag+和Mn2+)对缢蛏(Sinonovaculaconstricta)蛋白酶、淀粉酶和纤维素酶活力的影响。缢蛏壳长(5 64±0 25)cm,9种金属离子浓度分别设置为2×10-6mol/L、2×10-5mol/L、2×10-4mol/L、2×10-3mol/L、2×10-2mol/L5个梯度。实验表明,缢蛏3种消化酶活力大小为淀粉酶>蛋白酶>纤维素酶;在实验设置的浓度范围内,Cu2+、Fe3+、Mg2+、Pb2+对蛋白酶活力影响不显著,Hg2+、Zn2+、Ca2+、Ag+、Mn2+对蛋白酶活力影响显著;Mg2+、Pb2+对淀粉酶活力影响不显著,Cu2+、Fe3+、Hg2+、Zn2+、Ca2+、Ag+、Mn2+对淀粉酶活力影响显著;Ca2+、Mg2+、Zn2+对纤维素酶活力影响不显著,Pb2+、Cu2+、Fe3+、Hg2+、Ag+、Mn2+对纤维素酶活力影响显著。不同浓度金属离子对缢蛏蛋白酶、淀粉酶和纤维素酶活力的影响表现出不同的变化趋势。The concentrations of nine metal ions(Cu2+、Mg2+、Fe3+、Hg2+、Zn2+、Ca2+、Pb2+、Ag+ and Mn2+)were designed at 2×10-6 mol/L,　2×10-5 mol/L,　2×10-4 mol/L,2×10-3 mol/L and 2×10-2 mol/L, respectively, and the Sinonovacula constricta samples were employed with shell length at 4.25-6.85 cm. After 48 h starvation, the activities of protease, amylase and cellulase in the S. constricta were analyzed by using enzyme analytical method. The results indicate that the activity of amylase in S. constricta is the highest among the three digestive enzymes, and the activity of protease is higher than that of cellulase. Within the experimental concentration range, Cu2+,Fe3+,Mg2+ and Pb2+ have no significant effects, whereas Hg2+,Zn2+,Ca2+,Ag+ and Mn2+ have significant effects on protease activity of S. constricta; Mg2 and Pb2+ have no significant effects ,whereas Cu2+,Fe3+,Hg2+,Zn2+,Ca2+,Ag+ and Mn2+ have significant effects on amylase activity; Ca2+,Mg2+ and Zn2+ have no significant effects,whereas Pb2+,Cu2+,Fe3+,Hg2+,Ag+ and Mn2+ have significant effects on cellulase avtivity.The activities of protease, amylase and cellulase in S.constricta show different variation patterns under the different concentrations of metal ions.

关 键 词：金属离子 缢蛏 蛋白酶 淀粉酶 纤维素酶 

分 类 号：Q558.9[生物学—生物化学]